Measurement of functional fibrinogen levels using the Thrombelastograph - 13/08/11

Doi : 10.1016/j.jclinane.2007.09.017

Roger C. Carroll, PhD ^a,^⁎ : Professor and Director of Research, Robert M. Craft, MD ^a : Associate Professor, Jack J. Chavez, MD ^a : Associate Professor, Carolyn C. Snider, MT ^a : Medical Technologist, Ryan K. Kirby, BS ^a : Senior Research Technician, Eli Cohen, PhD ^b : Chief Executive Officer
^a Department of Anesthesiology, University of Tennessee Graduate School of Medicine, 1924 Alcoa Highway, Knoxville, TN 37920, USA
^b Haemoscope Corporation, 6231 West Howard Street, Niles, IL 60714-3404, USA

^⁎Corresponding author. Department of Anesthesiology, University of Tennessee Graduate School of Medicine, 1924 Alcoa Highway, Knoxville, TN 37920, USA. Tel.: +1 865 544 9469; fax: +1 865 525 5143.

Abstract

Study Objective

To validate a Thromboelastograph (Haemoscope Corporation, Niles, IL) assay for functional fibrinogen.

Design

Correlation study of the Thromboelastograph assay with two conventional fibrinogen assays by the standard Clauss method.

Setting

Research laboratory of a university medical center.

Participants and Interventions

Blood samples were obtained from 19 healthy volunteers.

Measurement and Main Results

Thromboelastograph assays, using heparinized whole blood from 19 healthy donors, indicated that reptilase-XIIIa mixture (Activator_f)–generated clot shear elasticity in dynes per square centimeter (G_f) correlated with fibrinogen (mg/dL). Blood from four donors was used to define the contribution of hematocrit (Hct) to G_f by titration with platelet-rich plasma. The G_f versus Hct gave linear correlations (r² = 0.746) with G_f = 1258 − 17.8 × % Hct. A commercial collection of 19 normal, 10 borderline, and one deficient for functional fibrinogen-citrated plasmas was assayed for G_f after recalcification using Activator_f. Of the 30 plasma samples, four were from factor X– or factor VII–deficient donors and one was from a coumadin-treated donor. There was a linear correlation of Activator_f G_f with functional fibrinogen (r² = 0.940) with G_f = −730 + 9.21 × fibrinogen (mg/dL).

Conclusion

Thrombelastography with Activator_f may be used to determine fibrinogen levels in whole blood.

Le texte complet de cet article est disponible en PDF.

Keywords : Thrombelastograph, Fibrinogen, Hematocrit

Plan

Introduction

Materials and methods

Materials

Human subjects

Thrombelastograph assays

Statistics

Results

Discussion

☆	Funding was provided by the Anesthesiology Research Fund, University of Tennessee, Knoxville, TN, and Haemoscope Corporation, Niles, IL.

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Vol 20 - N° 3

P. 186-190 - mai 2008 Retour au numéro

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Measurement of functional fibrinogen levels using the Thrombelastograph - 13/08/11

Abstract

Study Objective

Design

Setting

Participants and Interventions

Measurement and Main Results

Conclusion

Plan

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