Detection of spirochetal micro-organisms by focus-floating microscopy in necrobiotic xanthogranuloma - 19/08/11
, Klaus Eisendle, MD, PhD a, Christian Mensing, MD b, Bettina Zelger, MD c
Correspondence to: Bernhard Zelger, MD, MSc, Mag Prof Dr, Innsbruck Medical University, Department of Dermatology and Venerology, Anichstraße 35, AT-6020 Innsbruck, Austria.Abstract |
Background |
Necrobiotic xanthogranuloma (NXG) is a rare histiocytic disorder of unknown origin.
Objective |
We conducted an investigation of skin biopsy specimens from 7 patients with NXG for the presence of Borrelia by focus-floating microscopy.
Methods |
Focus-floating microscopy is a recently described, modified immunhistochemical technique in which the sections of a slide are simultaneously scanned both horizontally and vertically. Focus-floating microscopy is more sensitive for the detection of micro-organisms than polymerase chain reaction.
Results |
Borrelia could be detected as single, paired, or clusters of spirochetes in 6 cases of NXG whereas two cases investigated with a Borrelia-specific polymerase chain reaction (23s-RNA) remained negative.
Limitations |
Limited biopsy material in each patient prohibited a more detailed study of the life history of cutaneous lesions in NXG.
Conclusions |
The detection of this micro-organism in NXG points to a specific involvement of B burgdorferi or other similar strains in the development of or as a trigger of this disease.
Le texte complet de cet article est disponible en PDF.Abbreviations used : FFM, NXG, PCR
Plan
| Funding sources: None. |
|
| Conflicts of interest: None declared. |
|
| Reprints not available from the authors. |
Vol 57 - N° 6
P. 1026-1030 - décembre 2007 Retour au numéro
Article précédent
- Intractable wounds caused by calcific uremic arteriolopathy treated with bisphosphonates
- Takaaki Hanafusa, Yuji Yamaguchi, Mamori Tani, Noriko Umegaki, Yukari Nishimura, Ichiro Katayama
- Unusual variants of non-Langerhans cell histiocytoses
- Ruggero Caputo, Angelo Valerio Marzano, Emanuela Passoni, Emilio Berti
Bienvenue sur EM-consulte, la référence des professionnels de santé.
L’accès au texte intégral de cet article nécessite un abonnement.
Déjà abonné à cette revue ?