Molecular diagnosis of infectious diseases in dermatology - 21/08/11
Cleveland, Ohio, and Houston, Galveston, and Dallas, Texas
Abstract |
The molecular diagnosis of infectious disease has been growing considerably over the past decade. Nucleic acid amplification techniques, such as polymerase chain reaction, ligase chain reaction, transcription-mediated amplification, and nucleic acid sequence-based amplification, provide highly accurate diagnosis of numerous bacterial, viral, fungal, and parasitic infections involved in a variety of dermatologic diseases. In addition, signal amplification with hybrid capture, branched-DNA assays, and in situ hybridization have been used to detect numerous viral pathogens with high degrees of sensitivity and specificity. New technology that involves the use of DNA and protein microarrays has also enabled the detection of a variety of genes and gene mutations. With time, these diagnostic assays are decreasing in cost, gaining approval of the U.S. Food and Drug Administration, and becoming easier and more efficient to use. In the future, these assays will be able to deliver rapid and accurate diagnosis of infectious diseases within a single clinic visit.
Learning objective |
At the completion of this learning activity, participants should be familiar with molecular diagnosis of infectious diseases in dermatology.
Le texte complet de cet article est disponible en PDF.Abbreviations used : cDNA, cRNA, FDA, HHV, HPV, ISH, LCR, NASBA, PCR, rRNA, TMA
Plan
Funding sources: None. Conflict of interest: None identified. Some material in this article was presented in a lecture entitled “Diagnostic Update for Infectious Diseases” at the 61st annual meeting of the American Academy of Dermatology, San Francisco, 2003, by S. K. T. |
Vol 53 - N° 5
P. 749-765 - novembre 2005 Retour au numéroBienvenue sur EM-consulte, la référence des professionnels de santé.
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