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Journal of the American Academy of Dermatology
Volume 48, n° 4
pages 542-547 (avril 2003)
Doi : 10.1067/mjd.2003.99
accepted : 22 September 2002
Anti-epiligrin cicatricial pemphigoid and epidermolysis bullosa acquisita: Differentiation by use of indirect immunofluorescence microscopy

Robert M. Vodegel, MDa, Marcelus C.J.M. de Jong, PhDa, Hendri H. Pas, PhDa, Kim B. Yancey, MDb, Marcel F. Jonkman, MDPhDb
Groningen, The Netherlands, and Milwaukee, Wisconsin 
From the Departments of Dermatology, Groningen University Hospitala and Medical College of Wisconsin.b 


Binding of autoantibodies to laminin 5 and type VII collagen causes anti-epiligrin cicatricial pemphigoid and epidermolysis bullosa acquisita, respectively. Differentiation between these two dermal-binding autoimmune bullous dermatoses is not yet possible by indirect immunofluorescence microscopy. In this study we tested whether two recently described immunofluorescence techniques, “knockout” skin substrate and fluorescent overlay antigen mapping, can differentiate between anti-epiligrin cicatricial pemphigoid and epidermolysis bullosa acquisita. A total of 10 sera were tested: 4 with antilaminin 5, and 6 with antitype VII collagen autoantibodies, as characterized by either immunoblot or immunoprecipitation analysis. Differentiation between anti-epiligrin cicatricial pemphigoid and epidermolysis bullosa acquisita was possible in all 10 sera by indirect immunofluorescence using either knockout skin substrate or fluorescent overlay antigen mapping technique. (J Am Acad Dermatol 2003;48:542-7.)

The full text of this article is available in PDF format.

 Funding sources: Zon/MW.
 Conflict of interest: None identified.
 Reprints not available from authors.
 Correspondence: Prof Dr Marcel F. Jonkman, MD, Department of Dermatology, Groningen University Hospital, PO Box 30.001, 9700 RB Groningen, the Netherlands.
 0190-9622/2003/$30.00 + 0

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