Evaluation of a new paraffin-reactive CD7 T-cell deletion marker and a polymerase chain reaction-based T-cell receptor gene rearrangement assay: Implications for diagnosis of mycosis fungoides in community clinical practice - 02/09/11
Abstract |
Background: T-cell deletion and T-cell receptor (TCR) gene rearrangement studies are helpful in the early diagnosis and subsequent management of mycosis fungoides (MF). However, this often requires fresh-frozen tissue that can be difficult to obtain and evaluate in community clinical practice. A new CD7 antibody, the most sensitive and specific T-cell deletion marker, and a new TCR-γ gene rearrangement polymerase chain reaction (PCR) assay (TCR-γ PCR) are now available on routine paraffin-embedded biopsy specimens. Objective: Our purpose was to assess the utility of CD7 deletion and TCR-γ PCR in the diagnosis of MF using routine paraffin-embedded biopsy material. Methods: Cases of MF (n = 17) with matching frozen tissue immunohistochemistry and benign reactive dermatoses (lichen planus; n = 27) were assessed for CD7 (Clone: CD7-272) deletion and TCR-γ PCR using paraffin-embedded biopsy specimens. Results: Excellent concordance comparing frozen and paraffin embedded CD7 immunostaining (88%) was observed. CD7 deletion and TCR-γ PCR was sensitive (94%) and specific (96%) for a diagnosis of MF using paraffin-embedded biopsy specimens. Conclusion: In the diagnosis of MF, detection of CD7 deletion and monoclonal TCR rearrangements can be successfully performed in a cost-effective, timely fashion using routine formalin-fixed paraffin-embedded biopsy specimens. (J Am Acad Dermatol 2001;45:405-13.)
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Conflict of interest: None. |
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Reprint requests: Eric D. Hsi, MD, The Cleveland Clinic Foundation, 9500 Euclid Ave, L25, Cleveland OH, 44195. |
Vol 45 - N° 3
P. 405-413 - septembre 2001 Retour au numéroBienvenue sur EM-consulte, la référence des professionnels de santé.
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