Microphthalmia transcription factor immunohistochemistry: A useful diagnostic marker in the diagnosis and detection of cutaneous melanoma, sentinel lymph node metastases, and extracutaneous melanocytic neoplasms - 02/09/11
Abstract |
Background: Melanoma is the most lethal form of skin cancer. Diagnosis of amelanotic melanoma and detection of micrometastases in sentinel lymph nodes pose diagnostic and therapeutic dilemmas for the dermatopathologist and clinician. Objective: The purpose of this article is to determine the utility of immunohistochemistry using antibodies specific for microphthalmia in the identification of melanocytic lesions in the skin, eye, central nervous system, and sentinel lymph nodes. Methods: Paraffin-embedded, formalin-fixed specimens of cutaneous melanoma, including amelanotic melanoma and lentigo maligna melanoma, were stained with antibodies specific for microphthalmia. In addition, paraffin sections of extracutaneous lesions, including sentinel lymph nodes, uveal melanoma, and central nervous system melanocytomas, were stained with the specific microphthalmia antibody. Results: All cutaneous melanomas stained positively with microphthalmia, as did uveal melanomas and central nervous system melanocytomas. These findings confirm the melanocytic origin of melanocytomas and uveal melanomas and demonstrate that microphthalmia staining can be used to establish melanocytic origin of neoplasms. In addition, micrometastases were easily detected in sentinel lymph nodes. Conclusion: Microphthalmia transcription factor immunohistochemistry is a valuable tool in the identification of melanocytic lesions in numerous sites. Use of this stain may facilitate detection of micrometastases in sentinel lymph nodes. (J Am Acad Dermatol 2001;45:414-9.)
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Supported by the American Skin Association and National Institute of Arthritis, Musculoskeletal and Skin Disease grants AR44947, and AR02030, and the Emory Skin Disease Research Core Center grant P30 AR 42687. |
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Reprint requests: Jack L. Arbiser, MD, PhD, Department of Dermatology, Emory University School of Medicine, 5309 Woodruff Memorial Bldg, Atlanta, GA 30322. E-mail: jarbise@emory.edu. |
Vol 45 - N° 3
P. 414-419 - septembre 2001 Retour au numéroBienvenue sur EM-consulte, la référence des professionnels de santé.
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