Distinct pattern of immune cell population in the lung of human fetuses with cystic fibrosis - 03/09/11
Abstract |
Background: Airway inflammation and infection are early events in cystic fibrosis (CF) pathogenesis. The existence of an imbalance in the immune cell population of the CF fetal airway before infection remains completely unknown. Objective: The aim of this study was to determine whether early signs of inflammation are observed in CF airways during human fetal development. Methods: Tracheas and lungs were collected from 21 CF and 16 non-CF fetuses. In tissue sections, the numbers of neutrophils, mast cells, macrophages, and B and T lymphocytes were quantitatively analyzed by means of image cytometry. The presence of IL-4, IL-6, IL-8, IL-10, RANTES, IFN-γ, TNF-⍺, and NFκB and its inhibitor IκB-⍺ was qualitatively evaluated by immunofluorescent staining. Results: During fetal airway development, epithelial and glandular differentiation, as well as the distribution of inflammatory markers, was similar in CF and non-CF tissues. Significant differences between CF and non-CF fetal airways were observed only in the numbers of mast cells and macrophages. In the CF trachea, the mast cell number increased slowly but continuously, whereas in the non-CF trachea this number rapidly reached a plateau. In the CF lung, the macrophage number increased with time, whereas in the non-CF lung it decreased. Conclusion: Although no intrinsic inflammation was demonstrated, we observed a distinct appearance of mast cells and macrophages in CF airways in comparison with non-CF airways during fetal development. These 2 cell populations were greater in CF airways at a late stage of fetal development, suggesting their possible involvement in the early onset of inflammation in CF infants. (J Allergy Clin Immunol 2001;108:524-9.)
Le texte complet de cet article est disponible en PDF.Keywords : Cystic fibrosis, lung, human fetus, mast cell, macrophage
Abbreviations : CFTR:, IκB-⍺:, NFκB:, SCID:
Plan
Supported by Association Vaincre la Mucoviscidose. C. Hubeau is a doctoral fellow funded by Association Vaincre la Mucoviscidose. |
|
Reprint requests: Dominique Gaillard, MD, Faculté de Médecine, 51, rue Cognacq-Jay, 51100 Reims, France. |
Vol 108 - N° 4
P. 524-529 - octobre 2001 Retour au numéroBienvenue sur EM-consulte, la référence des professionnels de santé.
L’accès au texte intégral de cet article nécessite un abonnement.
Déjà abonné à cette revue ?