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Comptes Rendus Biologies
Sous presse. Epreuves corrigées par l'auteur. Disponible en ligne depuis le mercredi 7 septembre 2011
Doi : 10.1016/j.crvi.2011.07.005
Received : 30 April 2011 ;  accepted : 12 July 2011
Analysis of genetic variability in Aristaeomorpha foliacea (Crustacea, Aristeidae) using DNA-ISSR (Inter Simple Sequence Repeats) markers
 

Maria Victoria Fernández a, b, Ferruccio Maltagliati c, Federica G. Pannacciulli b, Maria Inés Roldán a,
a Laboratori d’Ictiologia Genètica, Universitat de Girona, Campus de Montilivi, 17071 Girona, Spain 
b Marine Environment Research Centre, ENEA–S. Teresa, PO Box 224, 19100 La Spezia, Italy 
c Dipartimento di Biologia, Università di Pisa, Via Derna 1, 56126 Pisa, Italy 

Corresponding author.
Abstract

This work reports the first genetic data of Aristaeomorpha foliacea , a marine decapod of high commercial value, from six Mediterranean localities and one new fishing ground in the Mozambique Channel. The use of five Inter Simple Sequence Repeat (ISSR) primers provided 150 polymorphic loci. Average estimates of genetic diversity did not significantly differ among sampled localities, with a mean value of heterozygosity H =0.105±0.015. Analysis of molecular variance (AMOVA) allocated>98% of genetic variability to the within-sample component, displaying values higher than those previously reported in ISSR studies on marine invertebrates. Cluster analyses did not detect geographically or genetically distinct groups. The observed lack of large-scale genetic differentiation is discussed in relation to the high potential for larval dispersal of the species and to features of the marker employed.

The full text of this article is available in PDF format.

Keywords : Marine decapod, Aristaeomorpha foliacea , Genetic variability, Gene flow, DNA-ISSRs




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