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Journal of the American Academy of Dermatology
Volume 38, n° 1
pages 56-60 (janvier 1998)
Doi : 10.1016/S0190-9622(98)70539-0
A new application of BCG antibody for rapid screening of various tissue microorganisms
 

Heinz Kutzner, MDa, Zsolt B. Argenyi, MDb, Luis Requena, MDc, Arno Rütten, MDa, Heino Hügel, MDa
Friedrichshafen, Germany, Iowa City, Iowa, and Madrid, Spain 

Abstract

Background: In routine dermatopathology there is growing demand for a simple, fast, cost-effective, and highly sensitive screening tool for the detection of microorganisms. Objective: Our purpose was to determine whether immunostaining with polyclonal anti–Mycobacterium bovis (BCG), which is known for its interspecies cross-reactivity, is a suitable screening method for many common microorganisms in dermatopathologic specimens. Methods: A total of 254 formalin-fixed, paraffin-embedded skin specimens of viral, protozoal, fungal, and bacterial infections were stained with appropriate histochemical stains and with anti-BCG. Results: Anti-BCG labeled bacteria and fungi with high sensitivity and minimal background staining, but did not react with spirochetes, viruses, or protozoa (Leishmania) . The quality and sensitivity of anti-BCG staining were superior to conventional histochemical stains. Conclusion: Because of its cross-reactivity with many bacteria and fungi as well as its high sensitivity and minimal background staining, the anti-BCG immunostain is a promising screening tool for the detection of the most common bacterial and fungal microorganisms in paraffin-embedded skin specimens. (J Am Acad Dermatol 1998;38:56-60.)

The full text of this article is available in PDF format.

 From Dermatohistologisches Gemeinschaftslabor, Friedrichshafen, a the Department of Pathology, University of Iowa, Iowa City,b and the Department of Dermatology, Fundación Jiménez Díaz, Universidad Autónoma, Madrid.c
 Reprint requests: Heinz Kutzner, MD, Dermatohistologisches Gemeinschaftslabor, Siemensstrasse 6/1, D-88048 Friedrichshafen, Germany.
 16/1/86989



© 1998  American Academy of Dermatology, Inc. Published by Elsevier Masson SAS@@#104157@@
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