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Archives of cardiovascular diseases
Volume 106, n° 1
pages 4-11 (janvier 2013)
Doi : 10.1016/j.acvd.2012.09.003
Received : 8 May 2012 ;  accepted : 4 September 2012
The effect of tissue factor pathway inhibitor on the expression of monocyte chemotactic protein-3 and IκB-⍺ stimulated by tumour necrosis factor-⍺ in cultured vascular smooth muscle cells
Effet de l’inhibition de la voie du facteur tissulaire sur l’expression de la protéine-3 et de IkB-⍺ chimiotactique stimulé par le TNF-⍺ dans des cultures de cellules vasculaires musculaires lisses
 

Figure 1




Figure 1 : 

Monocyte chemotactic protein-3 (MCP-3) messenger ribonucleic acid (mRNA) expression on the third day after gene transfer in different groups stimulated with tumour necrosis factor (TNF)-⍺ immediately after gene transfer (n =6). A. Representative autoradiographs of the MCP-3 polymerase chain reaction (PCR) products. B. Semiquantitative data regarding MCP-3 mRNA expression in vascular smooth muscle cells (VSMCs). Ad-LacZ: human recombinant adenovirus expressing bacterial β-galactosidase; Ad-TFPI: human recombinant adenovirus expressing tissue factor pathway inhibitor; DMEM: Dulbecco’s modified Eagle’s medium. *P <0.05.


Figure 2




Figure 2 : 

Monocyte chemotactic protein-3 (MCP-3) messenger ribonucleic acid (mRNA) expression on the third day after gene transfer in different groups stimulated with tumour necrosis factor (TNF)-⍺ 3 days after gene transfer (n =6). A. Representative autoradiographs of the MCP-3 polymerase chain reaction (PCR) products. B. Semiquantitative data regarding MCP-3 mRNA expression in vascular smooth muscle cells (VSMCs). Ad-LacZ: human recombinant adenovirus expressing bacterial β-galactosidase; Ad-TFPI: human recombinant adenovirus expressing tissue factor pathway inhibitor; DMEM: Dulbecco’s modified Eagle’s medium. *P <0.05.


Figure 3




Figure 3 : 

Western blotting was used to determine the expression of the IκB-⍺ protein in the cytoplasm of each group after stimulation with tumour necrosis factor (TNF)-⍺ at the third day after gene transfer (n =6). A. Western blotting result of IκB-⍺ protein expression. B. Semiquantitative data regarding IκB-⍺ protein expression. Ad-LacZ: human recombinant adenovirus expressing bacterial β-galactosidase; Ad-TFPI: human recombinant adenovirus expressing tissue factor pathway inhibitor; DMEM: Dulbecco’s modified Eagle’s medium. *P <0.05.


Figure 4




Figure 4 : 

Effect of tissue factor pathway inhibitor (TFPI) on the expression of IκB-⍺ after stimulation with tumour necrosis factor (TNF)-⍺ 3 days after gene transfer, by immunofluorescence method (×200) (n =7). Ad-LacZ: human recombinant adenovirus expressing bacterial β-galactosidase; Ad-TFPI: human recombinant adenovirus expressing TFPI; DMEM: Dulbecco’s modified Eagle’s medium.

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