Article

11 Iconography
Access to the text (HTML) Access to the text (HTML)
PDF Access to the PDF text
Advertising


Access to the full text of this article requires a subscription.
  • If you are a subscriber, please sign in 'My Account' at the top right of the screen.

  • If you want to subscribe to this journal, see our rates



Journal of the American Academy of Dermatology
Sous presse. Epreuves corrigées par l'auteur. Disponible en ligne depuis le mercredi 19 juin 2013
Doi : 10.1016/j.jaad.2013.04.052
accepted : 26 April 2013
Confocal laser scanning microscopy and optical coherence tomography for the evaluation of the kinetics and quantification of wound healing after fractional laser therapy
 

Elke Christina Erika Sattler, MD a, Katharina Poloczek b, Raphaela Kästle b, Julia Welzel, MD, PhD b,
a Department of Dermatology and Allergology at Ludwig-Maximilian University of Munich, Munich, Germany 
b Department of Dermatology and Allergology, General Hospital Augsburg, Augsburg, Germany 

Reprint requests: Julia Welzel, MD, PhD, Department of Dermatology and Allergology, General Hospital Augsburg, Sauerbruchstraße 6, 86179 Augsburg, Germany.
Abstract
Background

Information on the course of wound healing after fractional carbon-dioxide laser therapy has so far been gathered by histopathology. Confocal laser scanning microscopy (CLSM) and optical coherence tomography (OCT) allow the visualization of the upper layers of the skin in vivo over time.

Objective

The aim of this study was to determine whether CLSM and OCT can be used to quantify the kinetics of the dynamic wound-healing process.

Methods

Twenty healthy probands were treated with fractional carbon-dioxide laser with 8- and 16-W laser power, respectively. Optical follow-ups using CLSM and OCT were performed right after laser application and during the next 3 weeks.

Results

Although wound healing seems to be completed after day 7 to 14 clinically, on day 21 in 89.5% of the 8 W–treated areas and in 100% of the 16 W–treated areas subepidermal skin damage was still visible using CLSM and OCT imaging.

Limitations

The maximal penetration depth of CLSM is limited to the papillary dermis, whereas OCT can visualize deeper but with lower resolution.

Conclusion

In vivo CLSM and OCT are able to visualize changes after fractional laser treatment noninvasively. They allow especially the detection and quantification of substance defects and thereby help to elucidate the therapeutic effects.

The full text of this article is available in PDF format.

Key words : confocal laser scanning microscopy, fractional laser therapy, in vivo, noninvasive, optical coherence tomography, skin resurfacing, wound healing

Abbreviations used : CLSM, DEJ, MAZ, OCT



 The first two authors contributed equally to this article.
 Funding sources: None.
 Conflicts of interest: None declared.



© 2013  American Academy of Dermatology, Inc.@@#104156@@
EM-CONSULTE.COM is registrered at the CNIL, déclaration n° 1286925.
As per the Law relating to information storage and personal integrity, you have the right to oppose (art 26 of that law), access (art 34 of that law) and rectify (art 36 of that law) your personal data. You may thus request that your data, should it be inaccurate, incomplete, unclear, outdated, not be used or stored, be corrected, clarified, updated or deleted.
Personal information regarding our website's visitors, including their identity, is confidential.
The owners of this website hereby guarantee to respect the legal confidentiality conditions, applicable in France, and not to disclose this data to third parties.
Close
Article Outline