Differential gene expression in peripheral blood T cells from patients with psoriasis, lichen planus, and atopic dermatitis - 23/10/13
Abstract |
Background |
Psoriasis, lichen planus (LP), and atopic dermatitis (AD) are common chronic inflammatory skin diseases mediated by immune responses.
Objective |
We used RNA sequencing to investigate messenger RNA expression patterns in peripheral T cells of Chinese patients with psoriasis, LP, or AD and of healthy individuals.
Methods |
After peripheral T-cell proliferation, messenger RNA expression patterns were investigated by RNA sequencing, and 6 randomly selected genes were verified by real-time reverse transcriptase polymerase chain reaction.
Results |
Six genes were down-regulated and 33 were up-regulated in these diseases. Gene ontology analysis revealed enrichment of genes involved in positive regulation of T-cell activation. Regulation of nuclear premessenger RNA domain containing 1B (RPRD1B) expression was enhanced in psoriasis.
Limitations |
The role of hereditary factors in RPRD1B expression in T cells was not considered. Immunomodulators (thymopeptide, levamisole, BCG polysaccharide, nucleic acid injection, and transfer factor) were previously given to patients with psoriasis and LP, but not to patients with AD; the effects of these immunomodulators on gene expression is uncertain.
Conclusion |
RPRD1B may be involved in T-cell activation in our Chinese psoriatic cohort, and may play a role in stimulating epidermal hyperproliferation.
Le texte complet de cet article est disponible en PDF.Key words : atopic dermatitis, differentially expressed genes, lichen planus, psoriasis, regulation of nuclear premessenger RNA domain containing 1B, T cells
Abbreviations used : AD, cDNA, CD30, CD244, DEGs, GO, IL, KLRC2, LP, PCR, RGS16, RPRD1B, RT
Plan
| Supported by the National Natural Science Foundation of China (30972657 and 81271768). |
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| Conflicts of interest: None declared. |
Vol 69 - N° 5
P. e235-e243 - novembre 2013 Retour au numéro
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