Development of a novel noninvasive adhesive patch test for the evaluation of pigmented lesions of the skin - 16/07/14
, John P. Alsobrook, PhD c, Tara J. Palmer, MS c, Howard S. Robin, MD cAbstract |
Background |
The accurate clinical assessment of melanocytic neoplasms is a challenge for clinicians. Currently, obtaining a biopsy specimen and conducting a histologic examination is the standard of care. The incidence of melanoma in white populations is high, resulting in a large number of biopsy specimens.
Objective |
The objective of this study is to develop a noninvasive genomic method using mRNA to classify pigmented skin lesions as either benign or malignant.
Methods |
An adhesive patch method was used to obtain cells from the surface of melanocytic lesions. mRNA was extracted and a genomic signature was formulated in a training set of benign and malignant melanocytic neoplasms and subsequently tested in a validation set.
Results |
A 2-gene signature assessing the expression levels of CMIP and LINC00518 was able to differentiate melanomas from nevi in an independent validation set of 42 melanomas and 22 nevi with a sensitivity of 97.6% and specificity of 72.7%.
Limitations |
Larger and more diverse sets of melanomas and nevi are needed for additional validation of the molecular expression profiling in various subsets of melanocytic neoplasms.
Conclusion |
Our data suggest that mRNA molecular signatures can serve as a highly useful noninvasive method of differentiating melanoma from nevi and decrease the number of unnecessary biopsies.
Le texte complet de cet article est disponible en PDF.Key words : dysplastic nevi, melanocytic neoplasms, melanoma, molecular signatures, nevi
Abbreviations used : ACS, AUC, DTI, NF-κB, NNT, NPV, PPV, qRT-PCR, ROC
Plan
| Supported by DermTech International. |
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| Dr Gerami has served as a consultant to and received honoraria from Abbott Molecular Labs, Myriad Genetics, Castle Biosciences Inc, DermTech International, and Neogenomics. Drs Alsobrook and Robin are consultants to DermTech International. Ms Palmer is an employee of DermTech International. |
Vol 71 - N° 2
P. 237-244 - août 2014 Retour au numéro
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