Elemene, the essential oil of Curcuma wenyujin, inhibits osteogenic differentiation in ankylosing spondylitis - 10/03/15
, Nan Jiang a, Xing-hua Feng a, Xiao-yan Feng b, He-qiu Zhang b, Zhi-kui Wu a, Hui-ying Liang a, Quan Jiang a, Peng Chen aBienvenue sur EM-consulte, la référence des professionnels de santé.
L’accès au texte intégral de cet article nécessite un abonnement.
| pages | 4 |
| Iconographies | 3 |
| Vidéos | 0 |
| Autres | 0 |
Abstract |
Objective |
To explore the effects of Elemene, the essential oil of Curcuma wenyujin, on Bone morphogenetic protein/drosophila mothers against decapentaplegic proteins (BMP/SMADs) signal pathway in ankylosing spondylitis (AS) fibroblasts.
Methods |
Hip joint capsules were obtained from AS patients (n=10) receiving total hip replacement. Healthy hip joint capsules from patients with hip fracture (n=10) receiving surgery were included as a control. Primary fibroblast cell lines were established from these tissue samples. Fibroblasts were incubated with Elemene for 48hours. The protein expression was detected by Western blot. The mRNA expression was detected by real-time fluorescent quantitative polymerase chain reaction (PCR).
Results |
The results showed that the expression of proteins including SMAD1, pSMAD1, SMAD4 and Runt-related transcription factor 2 (RUNX2), and mRNA of RUNX2, which were over-expressed in AS fibroblasts were decreased in the AS fibroblasts cultured in medium with Elemene.
Conclusions |
Ele could have a hand in anti-osteogenic differentiation of AS fibroblasts by inhibiting the BMP/SMADs signal pathway and subsequently blocking expression of ossification marker genes RUNX2 that initiate the osteogenic differentiation.
Le texte complet de cet article est disponible en PDF.Keywords : Ankylosing spondylitis, Fibroblasts, Ossification, Elemene, BMP/SMADs
Abbreviations : AS, BMP, Ele, SMADs, PCR, RUNX2, TGF-β, TCM, DMEM, SD, PBS
Plan
Vol 82 - N° 2
P. 100-103 - mars 2015 Retour au numéro
Article précédent
- Analysis of associations between polymorphisms within genes coding for tumour necrosis factor (TNF)-alpha and TNF receptors and responsiveness to TNF-alpha blockers in patients with rheumatoid arthritis
- Jerzy Swierkot, Katarzyna Bogunia-Kubik, Beata Nowak, Katarzyna Bialowas, Lucyna Korman, Katarzyna Gebura, Katarzyna Kolossa, Slawomir Jeka, Piotr Wiland
- Effects of mud-bath therapy in psoriatic arthritis patients treated with TNF inhibitors. Clinical evaluation and assessment of synovial inflammation by contrast-enhanced ultrasound (CEUS)
- Franco Cozzi, Bernd Raffeiner, Valeria Beltrame, Luca Ciprian, Alessandro Coran, Constantin Botsios, Egle Perissinotto, Enrico Grisan, Roberta Ramonda, Francesca Oliviero, Roberto Stramare, Leonardo Punzi
Bienvenue sur EM-consulte, la référence des professionnels de santé.
L’accès au texte intégral de cet article nécessite un abonnement.
Bienvenue sur EM-consulte, la référence des professionnels de santé.
L’achat d’article à l’unité est indisponible à l’heure actuelle.
Déjà abonné à cette revue ?