‘Jardin de Granville’ is a new hybrid rose variety dedicated to cosmetic applications. To perform an exhaustive molecular investigation of the non-volatile secondary metabolites in this cultivar, a global approach was developed, combining four chromatographic techniques (HPTLC, HPLC-DAD-ELSD, UHPLC-HRMS and GC–MS). This approach afforded an on-line phytochemical fingerprinting of four plant organs of ‘Jardin de Granville’. Despite the wide diversity of molecular families and the pronounced differences in polarity between the molecules, this analytical strategy enabled an overview of the molecular composition of each sample to be rapidly obtained by HPTLC and HPLC and the molecular content to be correctly identified thanks to coupling with mass spectrometry. Polyphenols were identified in the EtOH/H₂O extracts; triterpenes, chlorophyll derivatives and lipids were characterized in the EtOAc extracts, and the fatty acids squalene, α-tocopherol and β-sitosterol were highlighted in the heptane extracts.