Safflower yellow promotes angiogenesis through p-VHL/ HIF-1α/VEGF signaling pathway in the process of osteogenic differentiation - 20/09/18
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Graphical abstract |
Highlights |
• | 1 SY promoted angiogenesis and osteogenic differentiation. |
• | 2 SY enhanced the expressions of angiogenesis and osteogenic differentiation markers. |
• | 3 SY promoted angiogenesis and osteogenic differentiation via enhancing HIF-1αlevel. |
• | 4 SY-induced angiogenesis and osteogenic differentiation via WSB-1/p-VHL pathway. |
Abstract |
Objective |
Safflower yellow (SY) is an active component ofCarthamus tinctorius L. that is widely used in orthopedics. This study aimed to evaluate the role of SY in angiogenesis and osteogenic differentiation.
Methods |
The migration and in vitro angiogenesis of SY (4.5, 9.0, 18 μg/ml)-treated human umbilical vein endothelial cells (HUVEC-12) were assessed by transwell and tube formation assay, respectively. Osteogenic differentiation ability was detected by alkaline phosphatase (ALP) and Alizarin Red S staining. The mRNA and protein expressions of related markers were determined by RT-qPCR and Western blot.
Results |
The migration and tube formation ability of HUVEC-12 were promoted by SY. Furthermore, SY facilitated the angiogenesis and osteogenic differentiation in the co-culture of HUVEC-12 and BMSCs by increasing hypoxia inducible factor-1α (HIF-1α), vascular endothelial growth factor (VEGF), Angiopoietin-2 (Ang-2), ALP, runt-related transcription factor 2 (Runx2) and osteopontin-1 (OPN-1) levels. Inhibition of HIF-1α expression by 3-(5-hydroxymethl-2-furyl)-1-benzylindazole (YC-1), restrained SY-induced proliferation, migration and angiogenesis of HUVEC-12 and the increased protein levels of VEGF, Ang-2, ALP, Runx2 and OPN-1. Finally, WD repeat and SOCS box-containing protein-1 (WSB-1)/Von Hippel-Lindau protein (p-VHL) pathway was involved in the beneficial effect of SY.
Conclusion |
SY promotes osteogenic differentiation via enhancing angiogenesis by regulating pVHL/HIF-1α/VEGF signaling pathway.
El texto completo de este artículo está disponible en PDF.Keywords : Angiogenesis, Osteogenic differentiation, p-VHL/ HIF-1α/ VEGF signaling pathway, Safflower yellow
Esquema
Vol 107
P. 1736-1743 - novembre 2018 Regresar al númeroBienvenido a EM-consulte, la referencia de los profesionales de la salud.
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