Phytocosmeceutical formulation development, characterization and its in-vivo investigations - 20/09/18
páginas | 12 |
Iconografías | 10 |
Vídeos | 0 |
Otros | 0 |
Graphical abstract |
The w/o emulsion loaded with 2% Prosopis cineraria extract rich in polyphenol, flavonoids and antioxidants was prepared. The polyphenols and flavonoids inhibit tyrosinase and PPAR- γ, which is a key enzyme in melanin synthesis and sebum production, respectively. Whereas, the anti-oxidants suppress the ROS production, proportional to the skin erythema. While, polyphenols and flavonoids have high affinity for skin collagen and elastin fibers that results in increased skin elasticity and moisturization.
Highlights |
• | Total phenolic, flavonoid, antioxidant, lipoxygenase, tyrosinase, anti-bacterial activity of stem, leaf and bark extract of Prosopis cineraria. |
• | In-vitro cytocompatibility of bark extract of Prosopis cineraria. |
• | Preparation of stable phytocosmetic emulsion formulation loaded with 2% pre-concentrated Prosopis cineraria bark extract. |
• | In-vitro rheological evaluation of formulation. |
• | Effect on skin melanin, erythema, sebum, hydration & elasticity levels post-treatment with phytocosmetic emulsion formulation. |
Abstract |
Several plants found rich in flavonoid, polyphenols, and antioxidants reported antiaging, oppose inflammation and carcinogenic properties but have rarely been applied in dermatology. The present study was an active attempt to formulate a stable phytocosmetic emulsion system loaded with 2% pre-concentrated Prosopis cineraria bark extract, aiming to revive facial skin properties. In order to obtain potent therapeutic activities, we first prepared extracts of stem, leaves, and bark and screen them on basis of phenolic, flavonoids contents and antioxidant, antibacterial, lipoxygenase and tyrosinase inhibition activities. Furthermore, cytocompatibility of the extract was also determined prior starting in vivo investigations. Then the in vivo performance of 2% bark extract loaded emulsion formulation was determined by using non-invasive probe cutometer and elastometer with comparison to base formulation. The preliminary experiment showed that bark extract has a significant amount of phenolic and flavonoid compounds with eminent antioxidant potential. Furthermore, indicated an efficient antibacterial, lipoxygenase, and tyrosinase enzyme inhibition activities. Importantly, the bark extract did not induce any toxicity or apoptosis, when incubated with HaCat cells. Moreover, the in vivo results showed the formulation (size 3 μm) decreased the skin melanin, erythema and sebum contents up to 2.1-,2.7-and 79%, while increased the skin hydration and elasticity up to 2-folds and 22% as compared to the base, respectively. Owing to enhanced therapeutic effects the phytocosmetic formulation proved to be a potential skin whitening, moisturizer, anti-acne, anti-wrinkle, anti-aging therapy and could actively induce skin rejuvenation and resurfacing.
El texto completo de este artículo está disponible en PDF.Keywords : Phytocosmetic emulsion, Phenolic and flavonoids, Tyrosinase inhibition, Biocompatibility, Skin rejuvenation and resurfacing
Esquema
Vol 107
P. 806-817 - novembre 2018 Regresar al númeroBienvenido a EM-consulte, la referencia de los profesionales de la salud.
El acceso al texto completo de este artículo requiere una suscripción.
Bienvenido a EM-consulte, la referencia de los profesionales de la salud.
La compra de artículos no está disponible en este momento.
¿Ya suscrito a @@106933@@ revista ?