Molecular identification of Fusarium species complexes: Which gene and which database to choose in clinical practice? - 29/03/19
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Abstract |
Identification of Fusarium at the level of the species complex is difficult with phenotypic methods, so it is necessary to use molecular sequencing methods. This study presents, for 33 isolates distributed among the four major species complexes, the performance of five identification schemes involving ITS (internal transcribed spacer), EF1α (translation elongation factor 1 alpha), RPB1 (largest subunit of RNA polymerase) and RPB2 (second largest subunit of RNA polymerase) genes and two databases: GenBank and Fusarium MLST (MultiLocus Sequence Typing). In our practice, the identification of the fungus from a culture is performed with EF1α and from a primary sample with ITS, using in both cases the specific database Fusarium MLST.
El texto completo de este artículo está disponible en PDF.Keywords : Fusarium species complex, Molecular identification, Fusarium MLST, EF1α
Esquema
Vol 29 - N° 1
P. 56-58 - avril 2019 Regresar al númeroBienvenido a EM-consulte, la referencia de los profesionales de la salud.
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