In ABO-incompatible bone marrow transplantation, an efficient depletion of red blood cells (RBC) within the graft is mandatory to avoid adverse events in transplanted patients. Using non therapeutic products, we evaluated the substitution of the standard density gradient-based separation (DGBS) over Ficoll-Paque with the use of an automated procedure intended for buffy coat only (SmartRedux software) introducing modifications within the settings to achieve a drastic reduction of the initial volume of the product. Both methods were conducted on the Sepax-2 device.

RBC depletion rates and CD34+ cells recoveries from eight procedures with SmartRedux software using “in-house” settings (method A) were compared to those obtained from four procedures using NeatCell software, an automated DGBS over Ficoll-Paque (method B).

Median erythrocyte depletion of 95,4% (92,7%–99,0%) and 99,8% (99,0%–99,9%) were observed using methods A and B, respectively. Median residual RBC volumes in the final product were 19 mL (4,4 mL–31,2 mL) and 0,7 mL (0,4 mL–4,7 mL), respectively (p = 0,014). CD34+ cells recoveries of 90,9% (62,7%–102,1%) and 78,4% (64,1%–86,2%) were achieved for methods A and B. Median platelet depletion was 16,6% (10%–42,7%) and 89,8% (88,5%-92,4%) using methods A and B, respectively (p = 0,004). Processing duration was shorter using method A (168 ± 29 min) than method B (295 ± 21 min) (p = 0,004).

Both methods achieved satisfactory erythrocyte depletion and CD34+ recovery. The use of Sepax-2 device in association with SmartRedux software could be extended to efficiently deplete RBC from large-volume BM in a raw instead of DGBS.