4-XL-PPD, a novel ginsenoside derivative, as potential therapeutic agents for gastric cancer shows anti-cancer activity via inducing cell apoptosis medicated generation of reactive oxygen species and inhibiting migratory and invasive - 18/09/19
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Highlights |
• | A dammarane derivative (4-XL-PPD) was obtained by structural modification. |
• | 4-XL-PPD showed much better anticancer activity than original compound AD-2 on cancer cells. |
• | 4-XL-PPD-induction of apoptosis of can be attenuated by the ROS scavenger N-acetylcysteine in gastric carcinoma cells. |
• | 4-XL-PPD inhibits migratory and invasive. |
Abstract |
(20R)-Dammarane-3β, 12β, 20, 25-tetrol (25-OH-PPD) is a ginsenoside isolated from Panax ginseng (C. A. Meyer). Previous research shows that the compound exhibits anti-cancer activities on many human cancer cell lines. In an attempt to enhance 25-OH-PPD activity, some derivatives were synthesized. Through screening of the derivative compounds for anti-cancer activity against gastric carcinoma cells, 12β-O-(L-Chloracetyl)-dammar-20(22)-ene-3β, 25-diol (4-XL-PPD) was selected as a strong anti-cancer agent. In this study, the anti-cancer mechanisms of 4-XL-PPD were investigated. The results showed that compound 4-XL-PPD resulted in a concentration-dependent inhibition of cells viability in gastric cancer cells, without affecting the viability of normal cell (human gastric epithelial cell line-GES-1). In BGC-803 cancer cells, 4-XL-PPD triggered apoptosis, and stimulated reactive oxygen species production. Apoptosis can be attenuated by the reactive oxygen species scavenger N-acetylcysteine. Meantime, 4-XL-PPD effectively suppressed the migratory and invasive capabilities of BGC-803 cancer cell and inhibited the expression levels of proteins associated with migratory and invasive capabilities (MMP-2, MMP-9, E-cadherin and CD34). All the results suggest that 4-XL-PPD exhibited remarkable anticancer activity base on inducing apoptosis via generating reactive oxygen species and inhibiting migratory and invasive, which support development of 4-XL-PPD as a potential agent for cancer therapy.
El texto completo de este artículo está disponible en PDF.Keywords : 4-XL-PPD, 25-OH-PPD, Apoptosis, Reactive oxygen species, Migratory and invasive
Esquema
Vol 118
Artículo 108589- octobre 2019 Regresar al númeroBienvenido a EM-consulte, la referencia de los profesionales de la salud.
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