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Genotyping of Candida albicans isolates from oropharyngeal candidiasis in head and neck cancer patients in Iran: Molecular epidemiology and SAP2 gene expression - 18/10/19

Doi : 10.1016/j.mycmed.2019.100896 
Z. Jahanshiri a, , S. Manifar b, F. Hatami c, F. Arastehnazar d, M. Shams-Ghahfarokhi e, M. Razzaghi-Abyaneh a
a Department of Mycology, Pasteur Institute of Iran, 1316943551 Tehran, Iran 
b Department of Oral Medicine, School of Dentistry, Tehran University of Medical Sciences, Cancer Institute of Tehran, Imam Khomeini Hospital Complex, Tehran, Iran 
c Department of Cellular and Molecular Biology, Tehran Medical Science Branch, Islamic Azad University, Tehran, Iran 
d Department of Biotechnology, Faculty of Biological Science, Alzahra University, Tehran, Iran 
e Department of Mycology, Faculty of Medical Sciences, Tarbiat Modares University, 14115-331 Tehran, Iran 

Corresponding author.
En prensa. Pruebas corregidas por el autor. Disponible en línea desde el vendredi 18 octobre 2019

Abstract

Objective

Multilocus sequence typing is a powerful method for genotyping of clinical isolates of Candida albicans. Cross-contamination between the patients is an important reason for nosocomial infections. Oropharyngeal candidiasis (OPC) caused by Calbicans is an important problem in patients with head and neck cancer, in Cancer Institute of Tehran. Here we studied the endemic genotypes of Calbicans isolates and the relationship between geographic distributions, potential cross-contaminations and the expression of SAP2 gene, an important gene in oral candidiasis, with MLST groups.

Material and methods

A total of 32 clinical strains of Calbicans isolated from head and neck cancer patients with oropharyngeal candidiasis were subjected to MLST analysis and SAP2 gene expression was analyzed by Real-time PCR.

Results

We identified 75 polymorphic sites in 7 loci of Calbicans isolates and 30 diploid sequence types which 27 of them were found as new. After eBURST analysis, our results determined that CC 124 was the most prevalent group among all CCs. SAP2 gene showed high expression in almost all OPC patients’ isolates, compared to the control.

Conclusion

We found few genetically-related as well as identical isolates among the 32 Candida strains which indicated low cross-contaminations among the patients. There was no relationship between Calbicans MLST profiles and their geographic distribution, cancer type and SAP2 gene expression. This lack of correlation was possibly due to the small understudy population; hence, finding more relevance requires studies with a higher number of samples.

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Keywords : Candida albicans, Multilocus sequence typing, Oropharyngeal candidiasis, Gene expression, SAP2


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© 2019  Publicado por Elsevier Masson SAS.
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