Dual-screening of anti-inflammatory and antioxidant active ingredients of shenxiang suhe pill and its potential multi-target therapy for coronary heart disease - 28/08/20
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Graphical abstract |
Highlights |
• | A total of 38 components of SXSHP were analyzed by UPLC/Q-TOF, including 18 components with high bioavailability. |
• | Dual-screening of anti-inflammatory and antioxidant was used to analyze the active ingredients of SXSHP. |
• | Three anti-inflammatory compounds were first reported. |
• | IL-15 and HIF-1 signaling pathways might be the mechanism of SXSHP in the treatment of CHD. |
Abstract |
Background |
Shenxiang Suhe Pill (SXSHP), a Chinese medicine formula, is widely used in clinic to treat coronary heart disease (CHD). However, due to the complex composition of SXSHP, its underlying mechanisms and pharmacodynamic properties are still unknown. In this paper, we try to define the compounds of SXSHP by dual-screening the active ingredients with anti-inflammation and antioxidant effects and predict its multi-target-pathway in CHD therapy using network pharmacology.
Methods |
The chemical constituents in SXSHP were analyzed by UPLC/Q-TOF. Then, the active ingredients with the anti-inflammation and antioxidant effects were dual-screened by in vitro experiments. Ingenuity pathway analysis (IPA) was used to analyze and predict the potential targets and pathways of the anti-inflammatory and antioxidant effects of SXSHP.
Results |
A total of 38 chemical constituents were identified in SXSHP, among which we screened six anti-inflammatory compounds: luteolin, isorhamnetin-3-O-beta-d-glucoside, 4-hydroxy-3-methoxycinnamaldehyde, benzoic acid, kaempferol-3-O-glucuronide acid, and blumeatin; and five antioxidant compounds: vanillin, eugenol, muscone, luteolin, and asiatic acid. IPA showed that eugenol, muscone, and 4-hydroxy-3-methoxycinnamaldehyde were closely related to the HIF-1 and IL-15 signaling pathways, which protect against oxidative stress and inflammation, respectively.
Conclusions |
Among the 38 ingredients in SXSHP, the anti-inflammatory pharmacological effects of isorhamnetin-3-O-beta-d-glucoside, blumeatin and 4-hydroxy-3-methoxycinnamaldehyde were reported for the first time. According to the network pharmacology analysis, eugenol, 4-hydroxy-3-methoxycinnamaldehyde and muscone are involved in the antioxidant HIF-1 pathway and the anti-inflammatory IL-15 pathway, and that may be the mechanism of SXSHP in the treatment of CHD.
El texto completo de este artículo está disponible en PDF.Abberviations : ALR, AMR, AR, ASI, BC, BEN, BLU, BNL, BZ, CF, CHD, CR, DMEM, DMSO, EUG, HIF-1, HMC, IHG, IL, IPA, KAE, LDH, LDL, LUT, MC, MMP, MPO, MTT, MUS, NF-κB, NO, OB, ROS, SEM, SOD, SR, SXSHP, TNF, UPLC/Q-TOF, VAN, VEGF
Keywords : Shenxiang suhe pill, Dual-screening, Anti-inflammation, Antioxidant, Network pharmacology, Coronary heart disease
Esquema
Vol 129
Artículo 110283- septembre 2020 Regresar al númeroBienvenido a EM-consulte, la referencia de los profesionales de la salud.
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