Circular RNA YAP1 attenuates osteoporosis through up-regulation of YAP1 and activation of Wnt/?-catenin pathway - 28/08/20
, Dong Yin ⁎ Bienvenido a EM-consulte, la referencia de los profesionales de la salud.
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Graphical abstract |
Abstract |
Background |
Osteoporosis is a systemic bone disease resulting from decreased bone mass and bone microstructure degeneration. Yes-associated protein 1 (YAP1) belongs to YAP family and plays a significant part in controlling bone quality.
Aim of the study |
Present study aimed to study the function and up-stream mechanism of YAP1 in the differentiation of BMSCs (bone marrow stromal cells) and MC3T3-E1.
Methods |
ALP staining, alizarin red staining and western blot analysis of osteogenic biomarkers determined osteogenic differentiation in BMSCs and MC3T3-E1. Mechanistic assays including luciferase reporter assay, RIP assay and RNA pull down assay disclosed the interplays between RNAs.
Results |
YAP1 promoted osteogenic differentiation of BMSCs and MC3T3-E1. Circ_0024097 originated from YAP1 sponged miR-376b-3p to elevate YAP1 expression in BMSCs and MC3T3-E1. Further, YAP1 mediated circ_0024097- promoted effects on osteogenic differentiation. Moreover, circ_0024097 activated Wnt/β-catenin pathway to facilitate osteogenic differentiation.
Conclusion |
It was firstly uncovered in present study that circ_0024097 attenuated osteoporosis through promoting osteogenic differentiation via miR-376b-3p/YAP1 axis and Wnt/β-catenin pathway.
El texto completo de este artículo está disponible en PDF.Keywords : Osteoporosis, YAP1, ceRNA, circRNA, Wnt/β-catenin pathway
Esquema
Vol 129
Artículo 110365- septembre 2020 Regresar al número
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Bienvenido a EM-consulte, la referencia de los profesionales de la salud.
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Bienvenido a EM-consulte, la referencia de los profesionales de la salud.
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