Therapeutic effect of 1,25(OH)2-VitaminD3 on fibrosis and angiogenesis of peritoneum induced by chlorhexidine - 28/08/20
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Highlights |
• | Heat shock protein 47(HSP47) can be induced by connective tissue growth factor(CTGF) in high glucose-treated peritoneal mesothelial cells. |
• | Marker of angiogenesis, CD34+/VEGF vessels was found in chlorhexidine gluconate induced peritoneal fibrosis (PF). |
• | Inhibition of CTGF/HSP47 and CD34/VEGF by VitD3 might be a novel treatment for PF via the regulation of collagen accumulation and angiogenesis. |
Abstract |
The biological activity of vitamin D, which mediated by the vitamin D receptor, is widespread throughout the body. The present study aimed to define whether 1,25-dihydroxy vitamin D3 (1,25-(OH)2D3) can protect against the progression of peritoneum fibrosis (PF) through its impact on the expression of connective tissue growth factor (CTGF) and heat shock protein 47 (HSP47) in vivo and in vitro. The male Sprague-Dawley (SD) rats of PF were induced by daily intraperitoneally injection of chlorhexidine gluconate (CG) for 4 wks. PF Rats were also treated with calcitriol (i.p. 6 ng/100g*d) from initiation of the CG. In calcitriol rats, the ultrafiltration and the ratio of dialysate urea nitrogen to blood urea nitrogen were improved (P < 0.05), pathological changes and peritoneal thickness were milder than that of the PF group. Calcitriol ameliorated high glucose-induced HSP47 expression in peritoneal mesothelial cells via CTGF down-regulation both at the mRNA level and protein level. Furthermore, calcitriol prevented angiogenic mediators of fibrosis by reduced the expression of CD34 and vascular endothelial growth factor (VEGF). The present study demonstrated that 1,25-(OH)2D3 intervention had a partially protective effect on peritoneum fibrosis, which might inhibit CTGF/HSP47 and CD34/VEGF in the peritoneum tissues.
El texto completo de este artículo está disponible en PDF.Keywords : Peritoneum fibrosis, 1,25-dihydroxy vitamin D3, Connective tissue growth factor, heat shock protein 47, Angiogenesis
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Vol 129
Artículo 110431- septembre 2020 Regresar al númeroBienvenido a EM-consulte, la referencia de los profesionales de la salud.
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