Candida nivariensis: Identification strategy in mycological laboratories - 10/09/20

Doi : 10.1016/j.mycmed.2020.101042

N. Cartier ^a, A. Chesnay ^a,^b, D. N’diaye ^c, C. Thorey ^c, M. Ferreira ^b,^d, O. Haillot ^e, É. Bailly ^a, G. Desoubeaux ^a,^b,^⁎
^a Parasitologie – mycologie – médecine tropicale, CHRU de Tours, 37044 Tours, France
^b CEPR – Inserm U1100/équipe 3, faculté de médecine, université de Tours, 37032 Tours, France
^c Médecine interne et maladies infectieuses, CHRU de Tours, 37044 Tours, France
^d Pneumologie, CHRU de Tours, 37044 Tours, France
^e Urologie, CHRU de Tours, 37044 Tours, France

^⁎Corresponding author. Pôle Biologie Médicale, 1^er étage du bâtiment B2a, service de parasitologie – mycologie – médecine tropicale, hôpital Bretonneau, CHU de Tours, 2, boulevard Tonnellé, 37044 cedex 9, France.Pôle Biologie Médicale, 1^er étage du bâtiment B2a, service de parasitologie – mycologie – médecine tropicale, hôpital Bretonneau, CHU de Tours2, boulevard Tonnellécedex 937044France

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Abstract

Candida nivariensis is a cryptic fungal species classified within the Candida glabrata complex. It was described for the first time in 2005 by the means of DNA sequencing. We report a rare case of C. nivariensis deep-seated infection occurring in a 77-year-old man hospitalized for cysto-prostatectomy. Phenotypic testing based on the direct examination and the macroscopic features of the in vitro culture initially suggested C. glabrata species, while MALDI-TOF mass spectrometry enables correct identification. The isolate was found resistant to fluconazole, like in almost 20% of the reported cases. Herein, we present our practical strategy to reliably characterize this rare cryptic species. To date, MALDI-TOF mass spectrometry-based analysis showed very good results for such a purpose.

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Keywords : Candida nivariensis, Candida glabrata, Candidemia, Candiduria, Candida bracariensis, Antifungal resistance