Effect of the acute and chronic administration of Lupinus albus β-conglutin on glycaemia, circulating cholesterol, and genes potentially involved - 19/12/20
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Graphical abstract |
Highlights |
• | Acute lupin β-conglutin treatment improves glycaemia response in normal rats. |
• | Chronic β-conglutin treatment prevents glycaemia deterioration in STZ-induced rats. |
• | β-conglutin treatment elicits a cholesterol lowering effect in STZ-induced rats. |
• | β-conglutin effects may involve antioxidant, antiinflammatory and estrogenic activity. |
Abstract |
Constituents of lupin seeds, like γ-conglutin and lupanine, have gained attention as potential complementary treatments for dysglycaemia management. Notwithstanding, the effect of other lupin components on carbohydrate metabolism, including β-conglutin protein, has received little attention. Here, we investigated the influence of the acute and chronic administration of β-conglutin on glycaemia modulation in normal and streptozotocin induced-to-diabetes rats. We analysed the liver transcriptome modulation exerted by β-conglutin in diabetes-induced rats using DNA microarrays to scout for potential molecular targets and pathways involved in this biological response. The acute administration of β-conglutin reduced the incremental area under the curve of glycaemia in normal and diabetes-induced animals. In a seven-day study with diabetic animals, glycaemia increased significantly in non-treated animals but remained unchanged in animals treated with a daily dose of β-conglutin. Total cholesterol was significantly lower at the end of the experimental period (−21.8 %, p = 0.039). The microarray and gene ontology analyses revealed several targets and pathways potentially modulated by β-conglutin treatment, including a possible down-regulation of Jun kinase activity. Moreover, our data indicate that targets related to oxidative stress, inflammation, and estrogenic activity might orchestrate these metabolic effects. In conclusion, our findings show that β-conglutin may help manage postprandial glycaemia and reduce cholesterol levels under the dysglycaemia stage. We identified and proposed new potential molecular targets for further research related to the mechanism of action of β-conglutin.
El texto completo de este artículo está disponible en PDF.Abbreviations : BW, DEG, DPP-IV, GO, iAUC, OGTT, OSTT, STZ, T2D
Keywords : Legumes, Nutraceuticals, Postprandial hyperglycemia, Glucose metabolism, Anticholesteremic agents, Gene expression profiling
Esquema
Vol 133
Artículo 110969- janvier 2021 Regresar al númeroBienvenido a EM-consulte, la referencia de los profesionales de la salud.
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