Capilliposide B blocks VEGF-induced angiogenesis in vitro in primary human retinal microvascular endothelial cells - 19/12/20

Doi : 10.1016/j.biopha.2020.110999

Haote Han ^a,^b,^c,^d,^{^{1
The authors Haote Han and Yanhui Yang have equally contributed to this work.}}, Yanhui Yang ^e,^{^{1
The authors Haote Han and Yanhui Yang have equally contributed to this work.}}, Zhipan Wu ^a,^b, Bing Liu ^c,^d,^f, Lijun Dong ^g, Hongwei Deng ^g,⁎ , Jingkui Tian ^a,^b,⁎ , Hetian Lei ^g,⁎
^a College of Biomedical Engineering & Instrument Science, Zhejiang University, Hangzhou, 310027, PR China
^b Zhejiang-Malaysia Joint Research Center for Traditional Medicine, Zhejiang University, Hangzhou, 310027, PR China
^c Schepens Eye Research Institute of Massachusetts Eye and Ear, Boston, MA, USA
^d Department of Ophthalmology, Harvard Medical School, Boston, MA, USA
^e School of Basic Medical Sciences, Ningxia Medical University, Yinchuan, Ningxia, PR China
^f Guangzhou Women and Children's Medical Center, Guangzhou Medical University, 510005, Guangzhou, PR China
^g Shenzhen Eye Hospital, Shenzhen Eye Institute, Jinan University, Shenzhen, Guangdong Province, PR China

^⁎Corresponding authors at: 18 Zetian Road, Shenzhen Eye Hospital, Futian District, Shenzhen, Guangdong Province, PR China.Shenzhen Eye HospitalFutian District18 Zetian RoadShenzhenGuangdong ProvincePR China^⁎⁎Corresponding author at: 148 Tianmushan Road, Zhejiang University, Hangzhou, Zhejiang province, PR China.Zhejiang University148 Tianmushan RoadHangzhouZhejiang provincePR China

Bienvenido a EM-consulte, la referencia de los profesionales de la salud.
El acceso al texto completo de este artículo requiere una suscripción.

páginas	7
Iconografías	7
Vídeos	0
Otros	0

Abstract

Abnormal angiogenesis is associated with intraocular diseases such as proliferative diabetic retinopathy and neovascular age-related macular degeneration, and current therapies for these eye diseases are not satisfactory. The purpose of this study was to determine whether capilliposide B (CPS-B), a novel oleanane triterpenoid saponin derived from Lysimachia capillipes Hemsl, can inhibit vascular endothelial growth factor (VEGF)-induced angiogenesis signaling events and cellular responses in primary human retinal microvascular endothelial cells (HRECs). Our study revealed that the capilliposide B IC₅₀ for HRECs was 8.5 μM at 72 h and that 1 μM capilliposide B specifically inhibited VEGF-induced activation of VEGFR2 and its downstream signaling enzymes Akt and Erk. In addition, we discovered that this chemical effectively blocked VEGF-stimulated proliferation, migration and tube formation of the HRECs, suggesting that capilliposide B is a promising prophylactic for angiogenesis-associated diseases such as proliferative diabetic retinopathy.

El texto completo de este artículo está disponible en PDF.

Keywords : Capilliposide B, Proliferative diabetic retinopathy, VEGF, VEGFR2, Akt, Erk, Migration, Proliferation, Tube formation

Esquema

Introduction

Materials and methods

Phase contrast imaging

Cell proliferation assay

Capilliposide B prevents VEGF-induced VEGFR2 signaling

CPS-B prevents the VEGF-induced proliferation of the HRECs

CPS-B blocks the VEGF-induced migration of the HRECs

CPS-B blocks VEGF-induced tube formation of the HRECs

Discussion

Author contribution statement

Exportación

Vol 133

Artículo 110999- janvier 2021 Regresar al número

Artículo precedente

SCAP knockout in SM22α-Cre mice induces defective angiogenesis in the placental labyrinth
Zhe Li, Danyang Li, Yuhan Rao, Li Wei, Mihua Liu, Guo Zheng, Yingcheng Yao, Xiaoli Hou, Yaxi Chen, Xiong Z. Ruan

| Artículo siguiente

Tamoxifen attenuates renal fibrosis in human kidney slices and rats subjected to unilateral ureteral obstruction
Stine Julie Tingskov, Michael Schou Jensen, Casper-Emil Tingskov Pedersen, Isabela Bastos Binotti Abreu de Araujo, Henricus A.M. Mutsaers, Rikke Nørregaard

Bienvenido a EM-consulte, la referencia de los profesionales de la salud.
El acceso al texto completo de este artículo requiere una suscripción.

Bienvenido a EM-consulte, la referencia de los profesionales de la salud.
La compra de artículos no está disponible en este momento.

¿Ya suscrito a @@106933@@ revista ?

conectar o crear una cuenta

Capilliposide B blocks VEGF-induced angiogenesis in vitro in primary human retinal microvascular endothelial cells - 19/12/20

Abstract

Esquema

Exportación citas

Fichero

Contenido

Mi cuenta

Aide & support

Declaración CNIL