Augmentation of anti-proliferative, pro-apoptotic and oxidant profiles induced by piceatannol in human breast carcinoma MCF-7 cells using zein nanostructures - 16/04/21
Bienvenido a EM-consulte, la referencia de los profesionales de la salud.
El acceso al texto completo de este artículo requiere una suscripción.
| páginas | 11 |
| Iconografías | 10 |
| Vídeos | 0 |
| Otros | 0 |
Abstract |
Piceatannol (PCT), a natural polyphenolic stilbene, has pleiotropic pharmacological potentials. It possesses cytotoxic activities toward variant cancerous cells. Zein nanospheres (ZN NSs) have been introduced as ideal nanostructures due to their natural origin, safety, histocompatibility. and convenient method of formulation. The purpose of this study was to explore the impact of PCT-ZN NSs formula on pharmacotherapy potential of PCT against human breast cancer MCF-7 cells. PCT-ZN NSs were formulated and characterized selectively to particle size, zeta potential, encapsulation efficiency and diffusion of PCT. The selected formula has a particle size of 84.4 ± 2.3 nm, zeta potential value of 33.8 ± 1.2 mV and encapsulation efficiency of 89.5 ± 4.1%. PCT-ZN NSs displayed significantly lower IC50 against MCF-7 cells by about 24 folds. Further, PCT-ZN NSs formula showed higher cellular uptake as compared to free PCT. Examination of cell cycle phases displayed cells accumulation in G2-M phase and increased percentage cells in pre-G1 phase indicating an apoptosis-enhancing activity. Annexin V staining indicated augmented early and late apoptosis. PCT-ZN NSs pro-apoptotic activity was confirmed by the observed significant increased mRNA expression of CASP3, p53, and Bax as well as decreased expression of Bcl2. In addition, PCT-ZN NSs induced oxidative stress as evidenced by depletion of glutathione reductase (GR) activity, increased generation of reactive oxygen species (ROS) and accumulation of lipid peroxidation products. Conclusively, ZN nanostructures of PCT revealed superior cell death-inducing activities against MCF-7 cells in comparison with free PCT. This is mediated, at least partly, by enhanced cellular uptake, pro-apoptotic activity, and oxidative stress potential.
El texto completo de este artículo está disponible en PDF.Graphical Abstract |
Highlights |
• | Piceatannol (PCT) was formulated in the form of zein (ZN) nanostructures (PCT-ZN NSs). |
• | PCT-ZN NSs exhibited enhanced cytotoxicity in human breast cancer cells MCF-7. |
• | PCT-ZN NSs caused accumulation of cells in G2-M phase and pre-G1 phases. |
• | PCT-ZN NSs enhanced apoptosis markers and induced oxidative stress. |
Keywords : MCF-7, Piceatannol, Nanospheres, Breast cancer, Zein, Apoptosis, Cell cycle, Diffusion, Cytotoxicity
Esquema
Vol 138
Artículo 111409- juin 2021 Regresar al número
Artículo precedente
- Epilobium angustifolium L. extract with high content in oenothein B on benign prostatic hyperplasia: A monocentric, randomized, double-blind, placebo-controlled clinical trial
- Cristina Esposito, Cristina Santarcangelo, Raffaello Masselli, Giuseppe Buonomo, Giovanna Nicotra, Violetta Insolia, Maria D'Avino, Giuseppe Caruso, Antonio Riccardo Buonomo, Roberto Sacchi, Eduardo Sommella, Pietro Campiglia, Gian Carlo Tenore, Maria Daglia
- PD-L1 silencing inhibits triple-negative breast cancer development and upregulates T-cell-induced pro-inflammatory cytokines
- Parisa Lotfinejad, Tohid Kazemi, Sahar Safaei, Mohammad Amini, Elmira Roshani asl, Elham Baghbani, Siamak Sandoghchian Shotorbani, Farhad Jadidi Niaragh, Afshin Derakhshani, Mahdi Abdoli Shadbad, Nicola Silvestris, Behzad Baradaran
Bienvenido a EM-consulte, la referencia de los profesionales de la salud.
El acceso al texto completo de este artículo requiere una suscripción.
Bienvenido a EM-consulte, la referencia de los profesionales de la salud.
La compra de artículos no está disponible en este momento.
¿Ya suscrito a @@106933@@ revista ?