Phloroglucinol and dieckol isolated from Ecklonia cava suppress impaired diabetic angiogenesis; A study of in-vitro and in-vivo - 16/04/21
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Abstract |
Abnormalities in angiogenesis that are associated with diabetes may contribute to vascular complications and result in disabilities and death. Furthermore, an imbalance in angiogenesis in different tissues, including the retina and kidney, can play a role in the pathogenesis of diabetic microvascular complications. Phlorotannins, such as phloroglucinol (PG) and dieckol (DK), which are found in Ecklonia cava exhibit antioxidant and anti-inflammatory activities that improve endothelial function in hypertension. However, reports on the effects of these compounds on diabetes-induced angiogenesis in vivo and in vitro are scarce. In this study, we assessed the antiangiogenic effects of PG and DK on endothelial cells treated with a high concentration of glucose to mimic angiogenesis. In addition, we sought to determine the effects of these compounds on cell proliferation, cell migration, and capillary formation. In silico docking of PG and DK into VEGFR-2 revealed their potential as therapeutic agents against angiogenesis. Further, both compounds were identified to inhibit the formation of the retinal vessel in transgenic zebrafish (flk:EGFP) embryos under high glucose conditions. These findings suggested that PG and DK derived from E. cava are potential inhibitors of angiogenesis in diabetic vascular complications and could, therefore, be used to develop angiogenic agents.
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Highlights |
• | PG and DK exert strong affinity for VEGFR-2, thereby blocking the VEGFR-2 pathway. |
• | PG and DK inhibit vital angiogenesis steps in high glucose-induced EA.hy926 cells. |
• | PG and DK regulate retinal vessel diameter in high glucose-induced zebrafish. |
• | PG and DK can be used as nutraceuticals for diabetes-related angiogenesis. |
Abbreviations : PG, DK, DR, VEGF, DMSO, PDB
Keywords : Phloroglucinol, Dieckol, Angiogenesis, Diabetes, EA.hy926 cells, Zebrafish
Esquema
Vol 138
Artículo 111431- juin 2021 Regresar al númeroBienvenido a EM-consulte, la referencia de los profesionales de la salud.
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