The toxicity mechanism of toxic compounds from Euphorbiae pekinensis Radix on zebrafish embryos - 16/04/21
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Abstract |
Euphorbiae pekinensis Radix (EP) is effective in treating various diseases, but it’s toxicity is a major obstacle in use in clinical. Although EP was processed with vinegar to reduce it’s toxicity, the detailed mechanism of toxicity in EP have not been clearly delineated. This study investigate the toxicity attenuation-mechanism of Euphorbiae pekinensis after being processed with vinegar (VEP) and the toxic mechanism of four compounds from EP on zebrafish embryos. The contents of four compounds decreased obviously in VEP. Correspondingly, slower development on embryos can be seen as some symptoms like reduction of heart rate, liver area and gastrointestinal peristalsis after exposed to the compounds. Some obvious pathological signals such as pericardial edema and yolk sac edema were observed. Furthermore, the compounds could increase the contents of MDA and GSH-PX and induce oxidative damage by inhibiting the activity of SOD. Also, four compounds could provoke apoptosis by up-regulating the expression level of p53, MDM2, Bax, Bcl-2 and activating the activity of caspase-3, caspase-9. In conclusion, the four compounds play an important role in the toxicity attenuation effects of VEP, which may be related to the apoptosis induction and oxidative damage. This would contribute to the clinical application and further toxicity-reduction mechanism research.
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Highlights |
• | A simultaneous determination of four compounds in EP and VEP using UPLC was established. |
• | Toxicity of them was evaluated on zebrafish embryos, and they possessed cardiotoxicity, liver and gastrointestinal toxicity. |
• | The toxicity mechanism was related to oxidative damage and the up-regulation of expression of apoptosis-associated genes. |
Abbreviations : Bax, Bcl-2, Caspase-3, Caspase-9, EP, GSH-PX, MDA, MDM2, OD, PE, qt-PCR, RBC, SI, SOD, SV-BA, TCM, VEP, YSE
Keywords : Euphorbiae pekinensis, Vinegar-processing, Toxicity, Zebrafish embryos, Apoptosis
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Vol 138
Artículo 111521- juin 2021 Regresar al númeroBienvenido a EM-consulte, la referencia de los profesionales de la salud.
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