Isothiocyanate Iberin inhibits cell proliferation and induces cell apoptosis in the progression of ovarian cancer by mediating ROS accumulation and GPX1 expression - 18/06/21
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Abstract |
Ovarian cancer (OC) is one of the most common gynecologic malignancies with poor survival rate, and Iberin is a member of isothiocyanate family with anti-tumor activity. However, the role of Iberin in OC development has not been reported yet. In this study, A2780 and OVCAR-3 cells were treated with gradient concentrations of Iberin to investigate the effect of Iberin on OC in vitro. Meanwhile, the in vivo tumorgenesis experiment was performed using female BALB/c nude mice treated with Iberin. Iberin inhibited cell proliferation, induced G2 cell cycle arrest and promoted cell apoptosis in OC cells. Besides, Iberin reduced GSH/GSSG level, enhanced ROS accumulation, and activated MAPK signaling in OC cells. More interestingly, ROS scavenger (NAC) compensated the anti-proliferative and pro-apoptotic effects of Iberin on OC cells, suggesting the involvement of ROS in the regulation of Iberin on OC cell growth. Notably, Iberin induced down-regulation of glutathione peroxidase-1 (GPX1), and over-expression of GPX1 reversed Iberin-mediated alterations in the proliferation, apoptosis and ROS accumulation of OC cells. The in vivo tumorgenesis study further evidenced the protection of Iberin against OC development. Besides, Iberin displayed a synergistic effect on the enhancement of chemo-sensitivity in OC cells. In summary, our study demonstrates the anti-tumor effect of Iberin on OC and its potential as a therapeutic agent against OC in the future.
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Highlights |
• | Iberin inhibits cell proliferation and induces cell apoptotic death in OC cells. |
• | Iberin plays anti-proliferative and pro-apoptotic role in OC cells by inducing ROS. |
• | Iberin mediates proliferation, apoptosis and ROS accumulation of OC cells by GPX1. |
• | Iberin inhibits the in vivo tumorgenesis of OC. |
• | Iberin promotes chemo-sensitivity of OC cells. |
Keywords : Iberin, Ovarian cancer, Cell proliferation, Apoptosis, ROS accumulation, GPX1
Esquema
Bienvenido a EM-consulte, la referencia de los profesionales de la salud.
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