Repeated topical exposure to staphylococcal enterotoxin B (SEB) elicits vigorous IgE antibody production and exacerbates allergen induced skin inflammation - 25/08/11
Abstract |
Rationale |
To explore the role of bacterial toxins in atopic dermatitis we investigated effects of topical application of SEB in the murine model of atopic dermatitis.
Methods |
BALB/c mice were epicutaneously treated with ovalbumin (OVA-group), SEB (SEB-group), combination of OVA and SEB (OVA/SEB-group) or vehicle (SAL-group). Cytokine and chemokine mRNA expression in the skin was investigated by real-time -PCR. Skin morphology was examined by histological methods. Total and specific antibody levels were studied by ELISA.
Results |
Total IgE levels were dramatically elevated in all SEB treated mice compared to OVA or SAL treated mice. SEB specific IgE levels were significantly higher in SEB-group compared to OVA/SEB-group. OVA-specific IgE levels were significantly higher in OVA/SEB-group compared with OVA treated mice. Topical SEB application elicited skin dermatitis and also significantly increased the degree of OVA induced skin inflammation in OVA/SEB-group. Significant induction of several cytokines and chemokines was found in the skin of all SEB treated mice compared to SAL-group. Synergistic induction of mRNA expression in OVA/SEB-group was seen in some cytokines and chemokines.
Conclusions |
Topical SEB application induces in mice a strong IgE antibody response and a mixed Th2/Th1 type skin inflammation. Cutaneous route exposure to SEB may critically regulate the development of skin dermatitis and the production of IgE antibodies in patients with atopic dermatitis.
El texto completo de este artículo está disponible en PDF. | Funding: Finnish Institute of Occupational Health |
Vol 113 - N° 2S
P. S97 - février 2004 Regresar al número
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