Autres domaines


Liver damage associated with exposure to aspirin and diazinon in male rats and the ameliorative effect of selenium - 10/05/14

Doi : 10.1016/j.biomag.2014.01.004 
Abdel-Tawab H. Mossa a, , Tarek M. Heikal a, Enayat Abdel Aziz Omara b
a Environmental Toxicology Research Unit (Etru), Pesticide Chemistry Department, National Research Centre, Tahrir Street, BO Box 12311, Dokki, Cairo, Egypt 
b Pathology Department, National Research Centre, Tahrir Street, BO Box 12311, Dokki, Cairo, Egypt 

Corresponding author. Tel.: +202 33371211; fax: +202 33370931.



The widespread use of organophosphorus insecticides (OPIs) consequently leads to the exposure of manufacturing workers, field applicators, the ecosystem, and finally the public to the possible toxic effects of OPIs. In addition, drugs or pharmaceutical products, which are used to cure diseases, are also xenobiotics with both therapeutic/toxic potentials. It is evident from the literature, which is very limited, that drug/insecticide interactions can result in altered response/toxicity, which is of clinical relevance. The aim of the present study was designed to assess the adverse effects of exposure to aspirin and diazinon and their combination on liver of male rats and hepatoprotective potential of selenium (Se).


Rats were oral administered with vehicle, acetyl salicylic acid (ASA) at the maximum administration dose (1350mg/personal/day=22.5mg/kg. b.wt.), diazinon (DIA) at 20mg/kg. b.wt. and Se at a dose of 200μg/kg b.wt./day and their combinations for 28 consecutive days. Serum liver biomarkers, e.g. ALT&AST, ALP, ChE, LDH, albumin, total protein were determined as well as histological and histochemical studies.


Body weight was statistically (P0.05) decreased, while relative liver weight was statistically (P0.05) increased in DIA and ASA+DIA-treated groups. The activities of serum aspartate aminotransferase (AST), alanine transaminase (ALT), alkaline phosphatase (ALP) and lactate dehydrogenase (LDH) were statistically (P0.05) increased, while the activity of cholinesterase (ChE) was decreased in rats exposed to DIA, ASA and DIA+ASA. In addition, administration of DIA, ASA and their combination resulted in damage of liver structures and increase in the immunoreactivity of caspase-3 expression in the cytoplasm of the hepatocytes as compared to the control group. Combination therapy with Se significantly (P0.05) restored these alterations to within the normal limits and prevents disruptions of liver structures.


The present study indicates that liver enzymes, histopathology and immunoreactivity of caspase-3 would trigger ASA- and DIA-induced liver injury. The severities of such observations were more pronounced in their combined exposure. Combination therapy with Se restored these alterations to within the normal limits and prevents disruptions of liver structures. The data throw light on the problem of simultaneous exposure to OPIs and commonly used drugs especially that are metabolised by CYP450. Accordingly, ASA should be avoided since many of the adverse effects associated with these drugs are similar to the complications of chronic liver disease especially of agricultural workers in developing countries, where the handling of drugs without medical prescription. We suppose that antioxidant supplementation may be beneficial for the people using ASA for longer periods.

El texto completo de este artículo está disponible en PDF.

Keywords : Acetyl salicylic acid, Diazinon, Liver enzymes, Histopathology, Immunohistochemistry antioxidant, Rat


© 2014  Elsevier Masson SAS. Reservados todos los derechos.
Añadir a mi biblioteca Eliminar de mi biblioteca Imprimir

    Exportación citas

  • Fichero

  • Contenido

Vol 4 - N° 2

P. 137-145 - avril 2014 Regresar al número
Artículo precedente Artículo precedente
  • Antiproliferative effect of coumarin by modulating oxidant/antioxidant status and inducing apoptosis in Hep2 cells
  • Sankaran Mirunalini, Krishnamoorthy Deepalakshmi, Jalagopal Manimozhi
| Artículo siguiente Artículo siguiente
  • In silico identification of potent inhibitors of alpha-synuclein aggregation and its in vivo evaluation using MPTP induced Parkinson mice model
  • Richard L. Jayaraj, Namasivayam Elangovan

Bienvenido a EM-consulte, la referencia de los profesionales de la salud.
El acceso al texto completo de este artículo requiere una suscripción.

¿Ya suscrito a @@106933@@ revista ?

Mi cuenta

Declaración CNIL

EM-CONSULTE.COM se declara a la CNIL, la declaración N º 1286925.

En virtud de la Ley N º 78-17 del 6 de enero de 1978, relativa a las computadoras, archivos y libertades, usted tiene el derecho de oposición (art.26 de la ley), el acceso (art.34 a 38 Ley), y correcta (artículo 36 de la ley) los datos que le conciernen. Por lo tanto, usted puede pedir que se corrija, complementado, clarificado, actualizado o suprimido información sobre usted que son inexactos, incompletos, engañosos, obsoletos o cuya recogida o de conservación o uso está prohibido.
La información personal sobre los visitantes de nuestro sitio, incluyendo su identidad, son confidenciales.
El jefe del sitio en el honor se compromete a respetar la confidencialidad de los requisitos legales aplicables en Francia y no de revelar dicha información a terceros.