Isolation of autologous adipose tissue-derived mesenchymal stem cells for bone repair - 26/10/16

Doi : 10.1016/j.otsr.2016.07.006

E. Raposio ^a , S. Bonomini ^b , F. Calderazzi ^c,^⁎
^a Department of surgical sciences, plastic surgery section, university of Parma, via Gramsci 14, 43100 Parma, Italy
^b Department of clinical and experimental medicine, division of hematology, Parma university hospital, via Gramsci 14, 43100 Parma, Italy
^c Department of surgical sciences, orthopedic clinic, Parma university hospital, via Gramsci 14, 43100 Parma, Italy

^⁎Corresponding author. Tel.: +0039 0521 704640; fax: +0039 0521 993030.

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Abstract

Introduction

Adipose tissue represents an abundant and accessible source of adult stem cells that can differentiate into cells and tissues of mesodermal origin, including osteogenic cells.

Methods

This paper describes the procedure to obtain a 5-cm³ saline sample, containing the adipose-derived stem cells (ASCs) pellet, starting from lipoaspirate obtained from a conventional abdominal liposuction.

Results

A mean of 2.5×10⁶ cells is isolated for each procedure; 35% (875000) of these are CD34+/CD45− cells, which express a subset of both positive (CD10, CD13, CD44, CD59, CD73, CD90, HLAABC) and negative (CD33, CD39, CD102, CD106, CD146, HLADR) cell-associated surface antigens, characterizing them as ASCs.

Conclusions

This procedure is easy, effective, economic and safe. It allows the harvesting of a significant number of ASCs that are ready for one-step bony regenerative surgical procedures.

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Keywords : Adipose-derived stem cells, Bone and cartilage, Cell therapy