Solid state NMR of salivary calculi: Proline-rich salivary proteins, citrate, polysaccharides, lipids, and organic–mineral interactions - 14/12/17
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Abstract |
Solid state NMR (ssNMR) can characterize mineral (31P) and organic (13C) components of human salivary stones (n = 8). All show apatitic 31P spectra. 13C ssNMR indicates more protein, of more consistent composition, than apatitic uroliths, with prominent signals from Tyr, Phe, and His. Citrate and lipids, identified by dipolar dephasing (DD), and polysaccharides are also observable in varying amounts. 13C{31P} rotational echo double resonance (13C{31P} REDOR) identifies carbon atoms in close (<ca. 0.5 nm) proximity to phosphorus and therefore probably binding with mineral. Citrate, sugar, and carboxylate signals exhibit strong 13C{31P} REDOR effects, also observed for signals between 50 and 60 ppm, from protein α-carbons and, possibly, phosphoserines and phospholipids, and sometimes for a 35–40 ppm envelope containing Asp–Cβ and Glu–Cγ signals. Amino acid analysis indicates high proline and aromatic content. 13C ssNMR and amino acid analyses are consistent with the preponderance of proline-rich salivary proteins such as statherin.
Le texte complet de cet article est disponible en PDF.Keywords : Apatite, Citrate, Polysaccharides, Proline-rich proteins, REDOR, Salivary proteins, Statherin
Plan
Vol 19 - N° 11-12
P. 1665-1671 - novembre 2016 Retour au numéroBienvenue sur EM-consulte, la référence des professionnels de santé.
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