LncRNA ASAP1-IT1 positively modulates the development of cholangiocarcinoma via hedgehog signaling pathway - 29/05/18
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Graphical abstract |
Highlights |
• | ASAP1-IT1 is upregulated in CCA tissues and cells. |
• | Knockdown of ASAP1-IT1 impedes cell proliferation, cell migration and EMT progress in CCA. |
• | ASAP1-IT1 activates hedgehog pathway through upregulating Smo and Gli1. |
• | ASAP1-IT1 improves CCA progression through activating hedgehog signaling pathway. |
Abstract |
Over the past decades, lncRNAs have attracted more and more attentions of researchers. It has been verified that lncRNAs can modulate multiple biological behaviors in various human cancers. LncRNA ASAP1-IT1 has been certified to be a tumor facilitator in several malignant tumors. This study aims to investigate the effects of dysregulated ASAP1-IT1 on biological processes of Cholangiocarcinoma. The high expression level of ASAP1-IT1 was tested in Cholangiocarcinoma tissues and cells with qRT-PCR. Upregulation of ASAP1-IT predicted the unfavorable prognosis for Cholangiocarcinoma patients. Next, ASAP1-IT1 was knocked down in cancerous cells for loss-of function assay. MTT, colony formation and transwell and western bot assays were performed to demonstrate the specific impacts of ASAP1-IT1 on proliferation, migration and EMT progression of Cholangiocarcinoma. Cells. As a results, the Cholangiocarcinoma progression was inhibited. Hedgehog signaling pathway has been discovered to be a treatment target in Cholangiocarcinoma. In this study, the interaction between ASAP1-IT1 and hedgehog pathway was specifically investigated. Smo and Gli1, two hedgehog-related proteins were examined in Cholangiocarcinoma cells. The results of qRT-PCR and western blot assay suggested that ASAP1-IT1 could positively modulate Smo and Gli1 in Cholangiocarcinoma. Finally, rescue assays were carried out to prove that ASAP1-IT1 could improve Cholangiocarcinoma progression and development via hedgehog signaling pathway.
Le texte complet de cet article est disponible en PDF.Keywords : CCA, ASAP1-IT1, Proliferation, Migration, Hedgehog signaling pathway
Plan
Vol 103
P. 167-173 - juillet 2018 Retour au numéroBienvenue sur EM-consulte, la référence des professionnels de santé.
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