Anti-inflammatory effect of the extracts from the branch of Taxillus yadoriki being parasitic in Neolitsea sericea in LPS-stimulated RAW264.7 cells - 11/06/18
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Graphical abstract |
Highlights |
• | TY-NS-B blocks NO and PGE2 production by inhibiting iNOS and COX-2 expression. |
• | TY-NS-B attenuates TNF-α and IL-1β expression. |
• | TY-NS-B inhibits NF-κB activating by inhibiting IκB-α degradation and subsequent p65 nucleus translocation. |
• | TY-NS-B inhibits p38 and JNK activation. |
• | TY-NS-B activates ATF3 expression. |
Abstract |
Mistletoe has been used as the herbal medicine to treat hypertension, diabetes mellitus, inflammation, arthritis and viral infection. In this study, we evaluated the anti-inflammatory effect of extracts of branch from Taxillus yadoriki being parasitic in Neolitsea sericea (TY-NS-B) using in vitro model. TY-NS-B significantly inhibited LPS-induced secretion of NO and PGE2 in RAW264.7 cells. TY-NS-B was also observed to inhibit LPS-mediated iNOS COX-2 expression. In addition, TY-NS-B attenuated production of inflammatory cytokines such as TNF-α and IL-1β induced by LPS. TY-NS-B blocked LPS-mediated inhibitor of IκB-α, and inhibited p65 translocation to the nucleus and NF-κB activation. Furthermore, TY-NS-B reduced the phosphorylation of MAPKs such as p38 and JNK, but not ERK1/2. In addition, TY-NS-B increased ATF3 expression and ATF3 knockdown by ATF3 siRNA attenuated TY-NS-B-mediated inhibition of pro-inflammatory mediator expression. Collectively, our results suggest that TY-NS-B exerts potential anti-inflammatory effects by suppressing NF-κB and MAPK signaling activation, and increasing ATF3 expression. These findings indicate that TY-NS-B could be further developed as an anti-inflammatory drug.
Le texte complet de cet article est disponible en PDF.Keywords : Anti-inflammation, Inflammatory response, Mistletoe, Taxillus yadoriki
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Vol 104
P. 1-7 - août 2018 Retour au numéroBienvenue sur EM-consulte, la référence des professionnels de santé.
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