Phoenix dactylifera protects against oxidative stress and hepatic injury induced by paracetamol intoxication in rats - 11/06/18
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Graphical abstract |
Highlights |
• | Paracetamol (PCM) induced adverse effects have been a notable concern world wide. |
• | We tested phoenix dactylifera (PLME & PLAE) effects for PCM-induced hepatotoxicity. |
• | PCM exerted a notable oxidative hepatotoxicity and increased serum liver enzymes. |
• | PLME & PLAE attenuate PCM-induced hepatotoxicity via blocking oxidative stress. |
• | Protective effects of PLME & PLAE were mediated by its potent antioxidant activity. |
Abstract |
The current studies were sought to determine effects of antioxidant potential of aqueous and methanolic extracts of Phoenix dactylifera leaves (PLAE and PLME) against the widely-used analgesic paracetamol (PCM) induced hepatotoxicity. Groups of rats were treated with or without PCM (1500 mg/kg), PLAE and PLME (300 mg/kg) and n-acetylcysteine (NAC, 50 mg/kg) followed by assessments of liver function tests, oxidative stress, antioxidant defenses, and hepatotoxicity. We observed that PCM significantly elevated serum liver markers, aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), gamma glutamyl transferase (GGT), and bilirubin compared to control (untreated) group. These PCM-induced effects were associated with oxidative stress as demonstrated by increased levels of malondialdehyde (MDA) and reduced levels of hepatic antioxidant enzymes, glutathione peroxidase (GPx), catalase (CAT), and superoxide dismutase (SOD). Pretreatment of PLME decreased ALT and AST by 78.2% and tissue MDA by 54.1%, and increased hepatic GPx (3.5 folds), CAT (7 folds) and SOD (2.5 folds) compared to PCM group. These PLME-mediated effects were comparable to NAC pretreatment. Histological analysis demonstrates that PLME conserved hepatic tissues against lesions such as inflammation, centrilobular necrosis, and hemorrhages induced by PCM. In contrast, PLAE-mediated effects were less effective in reducing levels of liver function enzymes, oxidative stress, and liver histopathological profiles, and restoring antioxidant defenses against PCM-induced intoxication. These findings indicate that PLME exerts protective effects against PCM-induced hepatotoxicity via scavenging free radicals and restoring hepatic antioxidant enzymes. Thus, PLME and its bioactive components could further be evaluated for their pharmacological properties against drug-induced deleterious effects.
Le texte complet de cet article est disponible en PDF.Abbreviations : AAP, ALP, ALT, AST, CAT, CYPs, DHBS, EDTA, GGT, GSH, GPx, H2O2, MDA, NAC, NAPQI, NO, O2–, OH–, ONOO–, PBS, PCM, PLAE, PLME, ROS, SOD, TBA
Keywords : Phoenix dactylifera, Leaves, Paracetamol, Hepatoprotective, Antioxidant, Free radicals, Phytochemicals
Plan
Vol 104
P. 366-374 - août 2018 Retour au numéroBienvenue sur EM-consulte, la référence des professionnels de santé.
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