RNA binding protein Lin28B confers gastric cancer cells stemness via directly binding to NRP-1 - 11/06/18
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Graphical abstract |
Highlights |
• | A new critical role of Lin28B in gastric cancer cells stemness is proposed. |
• | The mechanisms of Lin28B in gastric cancer cells stemness are further studied. |
• | A novel mode of regulation of NRP-1 by Lin28B in gastric cancer cells. |
• | The novel mechanism provides new potential targets for gastric cancer. |
Abstract |
This work aims to explore the roles and related mechanisms of RNA binding protein Lin28B in gastric cancer cells stemness. We found that Lin28B expression was negatively correlated with the overall survival (OS) of gastric cancer patients, and significantly increased in gastric cancer cells compared with that in gastric epithelial cells. Lin28B overexpression increased spheroid formation, expression of gastric cancer stemness-related markers, and decreased cisplatin sensitivity in gastric cancer cells. Mechanistically, Lin28B could directly bind to NRP-1 3′UTR, thus increasing NRP-1 mRNA stability and expression, and activate the downstream Wnt/β-catenin signaling. Knockdown of NRP-1 or treatment with Wnt/β-catenin antagonist could rescue the promotive effects of Lin28B on gastric cancer stemness. Thus, thes results indicate that Lin28B could facilitate gastric cancer stemness via directly binding to NRP-1 3′UTR and activating the downstream Wnt/β-catenin signaling.
Le texte complet de cet article est disponible en PDF.Keywords : Gastric cancer, Lin28B, NRP-1, Stemness, Wnt/β-catenin
Plan
Vol 104
P. 383-389 - août 2018 Retour au numéro
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