MiR-155 inhibits transformation of macrophages into foam cells via regulating CEH expression - 11/06/18
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Graphical abstract |
Highlights |
• | miR-155 upregulates mRNA and protein expression level of CEH in THP-1 macrophages. |
• | miR-155 inhibits the formation of foam cells, reduces lipid accumulation, and silencing CEH reverse its biological function. |
• | Tim-3 overexpression attenuates miR-155-mediated CEH induction. |
Abstract |
MiR-155 can inhibit the formation of atherosclerosis by interfering with the transformation of macrophages into foam cells that plays a critical role in the pathogenesis of atherosclerosis, but the precise mechanisms of miR-155 are still unknown. Herein, we observed that mRNA and protein expression levels of CEH were significantly upregulated in a dose- and time-dependent manner by transfected with miR-155 mimics in THP-1 macrophages. Further studies showed that overexpression of miR-155 can significantly inhibit foam cells formation, reduce intracellular CE accumulation and enhance the efflux of FC and cholesterol, result in a decrease of intracellular lipid accumulation; while this effect was significantly reversed by siCEH. Meanwhile, we found that Tim-3 is associated with miR-155-mediated CEH expression in THP-1 macrophage-derived foam cells. Overexpression of Tim-3 can attenuate miR-155-mediated CEH induction. Taken together, our findings demonstrated that miR-155 can inhibit the transformation of macrophages into foam cells by enhancing CEH signaling pathway in macrophages, this effect is likely to be achieved by inhibiting the expression of Tim-3.
Le texte complet de cet article est disponible en PDF.Abbreviations : CEH, As, ABCA1, TNF-a, IL-10, MIF, Tim-3, SR-A
Keywords : MiR-155, Cholesterol ester hydrolase, Macrophages, Tim-3
Plan
Vol 104
P. 645-651 - août 2018 Retour au numéroBienvenue sur EM-consulte, la référence des professionnels de santé.
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