Significant body mass increase by oral administration of a cascade of shIL21-MSTN yeast-based DNA vaccine in mice - 18/09/19
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Graphical abstract |
Highlights |
• | Oral myostatin specific yeast based DNA vaccines induced the immune responses in vivo. |
• | The body weight increased after oral administration of DNA vaccines. |
• | Validated the IL21 role in modulating the dendritic cells-mediated immune function in vivo. |
Abstract |
Base on the practical of MSTN-specific yeast-based protein vaccine in mice as described previously, this research was designed for developing a better DNA vaccine (a cascade of shIL21-MSTN yeast-based DNA vaccine) than solely MSTN yeast-based DNA vaccine to block the endogenous MSTN in the murine model. We first constructed the target vectors, including CMV-driven MSTN expression vector and a combined shIL21-MSTN vector which containing MSTN expression cassette and shIL21 (short hairpin RNA-IL21) expression cassette. After necessary validation, recombinant yeast vaccines harboring different vectors were well prepared. Subsequently, after 2-month administration, the MSTN-specific immune response was detected with western blots. The commercial ELISA assays indicated that the production of IL21 and IL6 were decreased compared with control groups. More importantly, the MSTN-specific antibody titer was much higher in the shIL21-MSTN group than MSTN group, which was consistent with the western blots result. The most important finding was significant body mass increased after oral administration of these yeast-based DNA vaccines, in which the shIL21-MSTN vaccine is slightly higher than the sole MSTN vaccine in mice. In this study, we confirmed the role of different MSTN-specific yeast-based DNA vaccines on increasing body mass in mice, to provide a good inspiration for livestock breeding through the new type of immunoregulatory method. On the other hand, we also detected the possible modulating role of shIL21 on the dendritic cell-mediated immune function which needs more practical application and deeper exploration.
Le texte complet de cet article est disponible en PDF.Keywords : Oral immunization, DNA vaccine, Saccharomyces cerevisiae, MSTN, Gene delivery, shIL21
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Vol 118
Article 109147- octobre 2019 Retour au numéroBienvenue sur EM-consulte, la référence des professionnels de santé.
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