Tetrahydropalmatine: Orchestrating survival – Regulating autophagy and apoptosis via the PI3K/AKT/mTOR pathway in perforator flaps - 04/12/23
Abstract |
Background |
Introduced in clinical practice in 1989, perforator flaps are vital for tissue defect repair, but they are challenged by distal necrosis. Tetrahydropalmatine (THP) from celandine is renowned for its anti-inflammatory and analgesic effects. This study investigates THP's use in perforator flaps.
Methods |
Thirty rats were divided into a control group and four THP concentration groups, while seventy-eight rats were categorized as control, THP, THP combined with rapamycin (RAP), and RAP alone. We created 11 cm by 2.5 cm multi-regional perforator flaps on rat backs, assessing survival blood flow and extracting skin flap tissue for autophagy, oxidative stress, apoptosis, and angiogenesis markers.
Results |
The THP group exhibited significantly reduced distal necrosis, increased blood flow density, and survival area on the seventh day compared to controls. Immunohistochemistry and Western blot results demonstrated improved anti-oxidative stress and angiogenesis markers, along with decreased autophagy and apoptosis indicators. Combining THP with RAP diminished flap survival compared to THP alone. This was supported by protein expression changes in the PI3K-AKT-mTOR pathway.
Conclusion |
THP enhances flap survival by modulating autophagy, reducing tissue edema, promoting angiogenesis, and mitigating apoptosis and oxidative stress. THP offers a potential strategy for enhancing multi-regional perforator flap survival through the PI3K/AKT/mTOR pathway. These findings highlight THP's promise in combatting perforator flap necrosis, uncovering a novel mechanism for its impact on flap survival.
Le texte complet de cet article est disponible en PDF.Graphical Abstract |
Highlights |
• | Tetrahydropalmatine, derived from a plant extract, exhibits a protective influence on ischemic flaps. |
• | Tetrahydropalmatine enhances flap survival while concurrently mitigating oxidative stress and apoptosis. |
• | Tetrahydropalmatine modulates autophagy and augments flap viability via the PI3K-AKT-mTOR pathway. |
• | Our findings illustrate that the inhibition of autophagy similarly fosters flap survival. |
Keywords : Multi-territory perforator flap, Tetrahydropalmatine, Angiogenesis, Autophagy, Apoptosis, Oxidative stress
Plan
Vol 169
Article 115887- décembre 2023 Retour au numéro
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