Anti-tumor effect of β-elemene in murine hepatocellular carcinoma cell line H22 depends on the level of c-Met downregulation - 06/06/12
Abstract |
Background |
β-elemene, extracted from ginger plant Rhizoma zeodaria exerts anticancer effect in a variety of tumor diseases including liver cancer by inhibiting tumor cells growth or promoting apoptotic cell death. c-Met is a receptor tyrosine kinase was widely found overexpressed in neoplastic tissues and participates in the cell proliferation, migration, invasion, survival. To explore the primary mechanism of action of β-elemene in cancer, we identified whether there are some relations between the expression level of c-Met and anticancer actions of β-elemene.
Methods |
The presence of c-Met in the H22 cells was confirmed by RT-PCR, Western blot and immunofluorescence analysis. In vitro, H22 cells were treated with β-elemene and the expression of c-Met was measured by RT-PCR, immunofluorescence analysis and Western blot. In vivo, an experimental H22 hepatocellular carcinoma (HCC) xenograft transplantation model, treating with different dose-dependent β-elemene, the expression of c-Met was measured by RT-PCR, Immunohistochemical staining assay.
Results |
c-Met more highly expressed in H22 cells than that in murine liver cells; after treating with β-elemene for 48h, the expression of c-met decreased markedly in H22 cells; furthermore, we showed that high-dosage groups significantly reduced tumor weights and down regulated the expression of c-Met compared to control groups in H22 transplanted tumor.
Conclusions |
Down-regulation of c-Met expression by β-elemene induces growth inhibition in murine hepatocellular carcinoma.
Le texte complet de cet article est disponible en PDF.Keywords : H22 cells, β-elemene, c-Met
Plan
Vol 2 - N° 2
P. 91-98 - avril 2012 Retour au numéroBienvenue sur EM-consulte, la référence des professionnels de santé.
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