CX3CR1 regulates osteoarthrosis chondrocyte proliferation and apoptosis via Wnt/β-catenin signaling - 15/12/17
, Feifei Wang, Xuehui Sun, Xiaoxue Wang, Lei Zhang, Yang LiBienvenido a EM-consulte, la referencia de los profesionales de la salud.
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Abstract |
Objective |
The study was aimed to investigate the impact of CX3CR1 expression on the proliferation and apoptosis of osteoarthrosis (OA) chondrocytes through Wnt/ β-catenin pathway.
Methods |
The expression levels of CX3CR1 and Wnt/β-catenin pathway-related genes mRNA and protein in OA chondrocytes were examined by qRT-PCR and western blot. MTT and flow cytometry (FCM) assays were employed to assess cell proliferation, cell cycle and apoptosis. XAV-939, a Wnt/β-catenin pathway inhibitor, was used to inhibit the pathway.
Results |
CX3CR1 was significantly overexpressed in OA cartilages than that in normal articular cartilages (P < 0.05). After siCX3CR1 transfection, the expression level of Wnt 3, nuclear β-catenin, Cyclin D1, MMP-13 and phosphorylated GSK-3β significantly increased, while cytoplasm β-catenin, GSK-3β and phosphorylated β-catenin expression was inhibited (P<0.05). XAV-939abolished the effects of siCX3CR1 on proliferation, apoptosis and cell cycle progression of OA chondrocytes (P<0.05).
Conclusions |
CX3CR1 regulated chondrocyte proliferation and apoptosis through Wnt/β-catenin signaling pathway. The overexpression of CX3CR1 in chondrocytes of OA may be closely related to the pathogenesis and progression of OA.
El texto completo de este artículo está disponible en PDF.Keywords : Osteoarthrosis, CX3CR1, Wnt/β-catenin, Proliferation, Apoptosis
Abbreviations : OA, TKA, DMEM, FBS, BCA, SDS-PAGE, PVDF, FCMF
Esquema
Vol 96
P. 1317-1323 - décembre 2017 Regresar al número
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Bienvenido a EM-consulte, la referencia de los profesionales de la salud.
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