Chronic exposure to arsenic, an inducer of oxidative stress, is one of the major causes of male infertility. Therefore, the present study investigated the protective role of Ellagic acid (EA), as a natural antioxidant, against testicular toxicity evoked by arsenic. Thirty-five male Wistar rats were divided into 5 treatment groups. Group 1 served as control, group 2 were orally exposed to sodium arsenite (SA, 10 mg/kg; 21 days), groups 3 and 4 were initially exposed to SA for 7 days and then were treated with both EA (10 and 30 mg/kg) and SA up to 21 days, and group 5 was treated with EA for 14 days. After this period, biochemical and histopathological parameters were evaluated in serum samples and testicular tissue. SA markedly reduced levels of serum testosterone, total antioxidant capacity, reduced glutathione as well as the activity of antioxidant enzymes. Furthermore, SA enhanced levels of malondialdehyde, tumor necrosis factor-α, interleukin-1β and nitric oxide in testes. Treatment with EA was found to reduce testicular arsenic accumulation and oxidative stress parameters. In addition, EA improved the serum testosterone level, testicular antioxidant markers and histological parameters after exposure to SA. EA may emerge as a promising therapeutic option to protect testes from arsenic-induced toxicity through reducing oxidative stress and inflammatory responses.