To identify changes in the adolescent mandibular canal (MC) using cone-beam computer tomography (CBCT) by locating three-dimensional (3D) anatomical landmarks in the mandible for later growth and treatment applications.

Two CBCT images taken 1.5–2 years apart for 50 patients (ages 11–17) were landmarked using AVIZO^®. Six core landmarks were placed relative to the right and left MCs. Three-dimensional coordinates (x, y, z) were obtained. Average distances between paired landmarks were measured using a paired samples t-test. Mean distance differences <1.50mm were determined clinically insignificant and stable. To determine landmark position consistency, repeat CBCTs from 11 patients were landmarked three times one week apart. Coordinates were determined and intra-class correlation coefficient statistics (ICC), mean error difference and confidence intervals were measured.

X and y-coordinates had an internal consistency of 1.00. Z-coordinates had a consistency of 0.995 or higher. Measurement error was <1mm. The largest mean distance change was 2.25mm±3.06mm between the right mandibular foramen and the landmark directly on the right canal, closest to the mesial root of the first molar. The smallest mean distance change was <0.01mm±1.7mm between the right and left mental foramina. Additionally, the bilateral mandibular foramina, bilateral distal and mesial roots of the first molar, and bilateral regions on the MC inferior to both distal and mesial roots of the first molar were stable.

All landmarks were determined reliable. The region between the right and left mental foramina was found most stable for this time period in adolescents.