Danshen can interact with intestinal bacteria from normal and chronic renal failure rats - 09/12/18
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Graphical abstract |
Highlights |
• | 16 biomarkers were found by 16S rRNA in normal and CRF rats intestinal bacteria. |
• | UPLC-QTOF/MS/MS was applied to determine the metabolites. |
• | Five metabolic pathways of tanshinones were identified. |
• | Three metabolic pathways of salvianolic acid were identified. |
• | Danshen interacts with intestinal bacteria from normal and CRF rats. |
Abstract |
Danshen (Salviae Miltiorrhizae Radix et Rhizoma, SMR) has been used as a traditional Chinese medicine in clinic for treatment of coronary heart diseases. Previous works have shown that the chronic renal failure (CRF) is closely related to changes of intestinal bacteria. The aim is to explore the interaction between active components of SMR and intestinal bacteria from normal and CRF rats. The changes of intestinal bacteria were evaluated among normal rats, CRF model rats and SMR-treated rats via 16S rRNA gene sequencing technology. UPLC-QTOF/MS was applied for the analysis and identification of metabolites.
Results |
Results showed that the following intestinal bacteria varied significantly in CRF rats, including Mucispirillum, Kurthia, Clostridium, Blautia, Butyrivibrio, Shuttleworthia, Peptococcus, Ruminococcus, Bradyrhizobium, Methylobacterium, Azospirillum, Thalassospira, Methylophilus, Pseudomonas, peptostreptococcaceae and bacteroidales. The ethanol extract of SMR (DS) significantly regulated Shuttleworthia, peptostreptococcaceae and Pseudomonas, while the water extract (DSS) significantly affected Peptococcus, peptostreptococcaceae and Ruminococcus. Methylation, demethylation, dehydrogenation, hydrogenation and hydroxylation were the major metabolic transformation of tanshinones in vitro by intestinal bacteria. Glucuronidation, methylation and hydrogenation were the main metabolic transformation of salvianolic acids. These results showed that the bioactive components of SMR, including tanshinones and salvianolic acids, might exert the medical effect via regulation intestinal bacteria.
El texto completo de este artículo está disponible en PDF.Abbreviations : CRF, SMR, MDF, DSS, DS, Z-group, M-group, DS-group, DSS-group, ZIBM, MIBM, PCA, PCoA, LEfSe, GAM
Keywords : Chronic renal failure, Salviae miltiorrhizae radix et rhizoma, Intestinal bacteria, Tanshinone, Salvianolic acid
Esquema
Vol 109
P. 1758-1771 - janvier 2019 Regresar al númeroBienvenido a EM-consulte, la referencia de los profesionales de la salud.
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