In vivo pathogenesis of colon carcinoma and its suppression by hydrophilic fractions of Clematis flammula via activation of TRAIL death machinery (DRs) expression - 09/12/18
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Highlights |
• | Characterization of hydrophilic fraction of Clematis flammula aerial parts with Gas Chromatography and Mass Spectrometry/ cytotoxicity of Clematis flammula hydrolate towards human colon cancer cells (HCT-116)/Radical oxygen species production after treatment with hydrolate of Clematis flammula/Clematis hydrolate synergizes the effect of Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL)/Gene expression. |
Abstract |
This work focused on characterizing hydrophilic fractions of Clematis flammula (CFl). The data here clearly demonstrated that hydrolate fractions act as a free radical scavengers and inhibited proliferation of different cell lines in a time- and concentration-dependent manner, transwell, and with a significant cytotoxic effect. Treating cells with CFl had the effect of suppressing cell growth attenuated by ROS generation in colonic carcinoma. Moreover, CFl in HCT116 cells suppressed survival, proliferation, invasion, angiogenesis and metastasis in vitro by inhibiting gene expression. Following CFl treatment, caspases and PARP cleavage were detected. The up- and down-regulated genes obtained from the WBA of the effect of CFl showed that several biological processes were associated with apoptosis and induction of G1 cell cycle arrest. CFl synergizes the effect of TRAIL by down-regulating the expression of cell survival proteins involved in apoptosis compared to cells treated with CFl or TRAIL alone. Our findings showed that CFl sensitizes apoptosis in TRAIL-resistant cells by activating MAPKs, SP1, and CHOP, that induced DR5 expression. Overall, our data showed that CFl is a promising antitumor agent through kinases and transcription factor induction, both of which are required to activate TRAIL receptors. Colon inflammation induced by LPS was inhibited by CFl hydrolate.
El texto completo de este artículo está disponible en PDF.Abbreviations : CHOP, JNK, DCF-DA, DMSO, DR, ERK, MAPKs, TRAIL, LPS, 5-Fu
Keywords : Clematis flammula, TRAIL, Death receptors, Decoy receptors, MAPKs, Transcription factor, Gene expression
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Vol 109
P. 2182-2191 - janvier 2019 Regresar al númeroBienvenido a EM-consulte, la referencia de los profesionales de la salud.
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