SNHG14 confers gefitinib resistance in non-small cell lung cancer by up-regulating ABCB1 via sponging miR-206-3p - 16/06/19
páginas | 9 |
Iconografías | 7 |
Vídeos | 0 |
Otros | 0 |
Graphical abstract |
Highlights |
• | SNHG14 expression is increased in gefitinib-resistant NSCLC tissues and cells. |
• | Knockdown of SNHG14 sensitizes NSCLC cells to gefitinib in vitro and in vivo. |
• | miR-206-mediated gefitinib sensitivity is reversed in NSCLC cells by SNHG14 overexpression. |
• | SNHG14 suppresses miR-206 expression as a molecular sponge, thereby increasing ABCB1 expression. |
Abstract |
Gefitinib, an epidermal growth factor receptor tyrosine kinase inhibitor (EGFR-TKI), has been widely used as a first-line agent in EGFR-mutant non-small cell lung cancer (NSCLC). Nevertheless, the development of chemoresistance ultimately limited the curative effect of anti-cancer drugs. The present study aims to investigate the functions of SNHG14 in gefitinib resistance and gain insight into the underlying molecular mechanisms. In the present study, we found that SNHG14 expression was elevated and miR-206-3p expression was decreased in gefitinib-resistant NSCLC tumor tissues and cells. Functionally, SNHG14 overexpression increased gefitinib resistance by promoting cell viability, lowering apoptosis and enhancing colony forming ability, while SNHG14 knockdown reduced gefitinib resistance in NSCLC cells. Mechanistically, SNHG14 induced ABCB1 expression via interaction with miR-206-3p. Moreover, depletion of SNHG14 enhanced the sensitivity of NSCLC cells to gefitinib in vivo. Together, SNHG14 confers gefitinib resistance in NSCLC by regulating miR-206-3p/ABCB1 pathway, contributing to a better understanding of SNHG14 in acquired resistance and elucidating a candidate target to improve treatment response of NSCLC patients.
El texto completo de este artículo está disponible en PDF.Keywords : SNHG14, miR-206-3p, ABCB1, NSCLC, Gefitinib
Esquema
Vol 116
Artículo 108995- août 2019 Regresar al númeroBienvenido a EM-consulte, la referencia de los profesionales de la salud.
El acceso al texto completo de este artículo requiere una suscripción.
Bienvenido a EM-consulte, la referencia de los profesionales de la salud.
La compra de artículos no está disponible en este momento.
¿Ya suscrito a @@106933@@ revista ?