Correlation analysis between virtual and Complement-Dependent-Cytotoxicity crossmatch in a monocenter retrospective series of 118 allografted patients - 27/05/21
, Sonia Lo Po' a, Daniela Lumia a, Giuliana Lando a, Giorgia Cornacchini a, Lara Crucitti b, Maria Teresa Pugliano a, Elisabetta Volpato a, Irene Cuppari a, Elisabetta Sommaruga a, Maria Grazia Pipitone a, Sara Labate a, Giovanni Grillo c, Elisa Zucchetti c, Silvano Rossini aBienvenido a EM-consulte, la referencia de los profesionales de la salud.
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Abstract |
Purpose of the Study |
The detection of patients' anti-HLA antibodies before allogeneic hematopoietic stem cell transplantation (HSCT) may affect post-transplant outcome, due to a potential detrimental impact on engraftment or toxicity-related issues. Crossmatch (XM) techniques provide support to physicians during the pre-transplant phase but the role of Complement-Dependent Cytotoxicity XM (CDC-XM) is not well-defined when performed routinely and in parallel with the virtual XM.
Patients and Methods |
We report here our experience with both virtual and CDC-XM tests on n = 118 patients undergoing search for a donor other than HLA-identical sibling from July 2013 to June 2018 at our Institution. When anti-HLA antibodies (Abs) were present, they were classified as donor-specific Abs (DSA) or non-DSA.
Results |
On the n = 118 patients, n = 35 (29.7 %) had a positive virtual XM test (of which one of more DSA were found in n = 8; 6.8 %) and n = 5 had a positive CDC-XM test. These latter, positive for HLA class II only, were interpreted as false-positive results due to prior administration of anti-CD20 to the patients, all affected by lymphoma; none of them had a positive virtual XM for anti-HLA Abs of class II. Importantly, all these patients successfully engrafted, further supporting the lack of significant impact of CDC-XM positive results in this series.
Conclusions |
According to our data on more than a hundred patients, routinely performed CDC-XM does not seem to add significant information with respect to virtual XM. We cannot exclude the usefulness of CDC-XM in specific situations, although a positive CDC-XM result was an unfrequent event.
El texto completo de este artículo está disponible en PDF.Abbreviations : HSCT, XM, CDC-XM, Abs, DSA, HLA, FC-XM, C1q, MFI, CMV
Keywords : Hematopoietic stem cell transplantation, Crossmatch, Donor, Anti-HLA antibodies, Donor-specific antibodies
Esquema
Vol 69 - N° 2
Artículo 103287- mai 2021 Regresar al número
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